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Tris Acetate-EDTA buffer

SIGMA/T8280 - 10× concentrate, BioReagent, Molecular Biology, DNase and RNase, none detected, powder blend, suitable for electrophoresis

Synonym: TAE buffer

MDL Number: MFCD00236357
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-T8280-1L 1 L
 $77.20
1/EA		 Add To Favorites

 

application(s) diagnostic assay manufacturing
form powder blend
grade Molecular Biology
impurities DNase and RNase, none detected
InChI 1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
InChI key HGEVZDLYZYVYHD-UHFFFAOYSA-N
pH 8.1-8.5
product line BioReagent
Quality Level 200 
SMILES string CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
suitability suitable for electrophoresis
  suitable for gel electrophoresis (after dilution to working concentration)
Application: Ready for use in gel electrophoresis after dilution to working concentrations.
Application: TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Packaging: 1 L in poly bottle
Packaging: Packaged in plastic bottles large enough to contain the concentrate after reconstitution.
Preparation Note: Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).
Preparation Note: Produces a 10× concentrate (0.4 M Tris-acetate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
UNSPSC 41105319

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