Anti-β-Tubulin antibody, Mouse monoclonal
SIGMA/T8328 - ~2.0 mg/mL, clone AA2, purified from hybridoma cell culture
Synonym: Beta Tubulin Antibody Sigma; Beta Tubulin Antibody Sigma - Monoclonal Anti-β-Tubulin antibody produced in mouse; Anti-TUBB; Anti-Tubulin, beta
MDL Number: MFCD00145892
Product Type: Chemical
Western Blotting JOURNAL CITATION: G9a-mediated methylation of ERα links the PHF20/MOF histone acetyltransferase complex to hormonal gene expression. By: Zhang, X., Peng, D., et al. in Nat Commun, 2016. PubMed ID: 26960573 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/doifinder/10.1038/ncomms10810), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: G9a-mediated methylation of ERα links the PHF20/MOF histone acetyltransferase complex to hormonal gene expression. By: Zhang, X., Peng, D., et al. in Nat Commun, 2016. PubMed ID: 26960573 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/doifinder/10.1038/ncomms10810), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence
HeLa cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 5 μg/mL Monoclonal Anti-β-tubulin Clone: AA2 (Cat. No. T8328). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
HeLa cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 5 μg/mL Monoclonal Anti-β-tubulin Clone: AA2 (Cat. No. T8328). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-β-tubulin Clone: AA2 (Cat. No. T8328). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Neuro-2a 2. HeLa 3. HEK- 293T 4. Jurkat 5. Rat brain 6. COS7 7. NIH- 3T3 8. NRK 9. PC12
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AA2, monoclonal |
| concentration | ~2.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~50 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat, bovine |
| storage temp. | −20°C |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 1-2 μg/mL using Neuro-2a cell extract | |
| UniProt accession no. | P07437  |
| Application: | Anti-Tubulin βIII antibody has been used in western blot analysis. |
| Application: | Monoclonal anti-β-tubulin antibody (diluted 1: 5000) can be used as a primary antibody in immunofluorescence staining of mammalian oocytes. It can also be used in numerous immunochemical techniques like-, immunocytochemistry, immunoblotting (50kD approx), immunohistochemistry and immunoprecipitation. |
| Application: | Monoclonal Anti-β-Tubulin antibody produced in mouse has been used in western blotting and immunofluorescence staining. |
| Biochem/physiol Actions: | Proper organization of microtubules mediated by tubulin is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. |
| Biochem/physiol Actions: | Upregulation of tubulin βIII (TUBB3) shows vigorous tumor characteristics and genetic instability in urinary bladder cancer. It participates in the separation of chromosome. Individuals with congenital fibrosis of the extraocular muscle type 3 (CFEOM3) show mutations in TUBB3. βIII-tubulin modulates glucose metabolism, thereby transforms glucose starvation stress signaling to develop the multiplication of cell and survival in non-small cell lung cancer cells. |
| Biochem/physiol Actions: | β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP). B2702 peptide binds to β-tubulin and inhibits natural killer (NK) cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Tubulin βIII (TUBB3) is a constituent of microtubules. It is expressed in mature neurons. It is located on human chromosome 16q24. |
| General description: | Tubulin, a 100kDa protein is present in all cells as a heterodimer of two similar polypeptides α and β, which assemble to form microtubules. Monoclonal anti-β-tubulin antibody can be used to visualize HRP signal using enhanced chemiluminescence. |
| General description: | β-tubulin belongs to the subfamily of tubulins, which are the major building blocks of microtubules. β-tubulin has a molecular weight of 55kDa. β-tubulin structure is characterized with core of two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site and a C-terminal domain that contains the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βII (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V) and βVI (class VI)). |
| Immunogen: | Purified bovine tubulin |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Mouse anti-β-tubulin antibody reacts specifically with β tubulin, types I, II, III, and IV of bovine, rat, mouse and human. |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |