Anti-β-Tubulin II antibody, Mouse monoclonal
SIGMA/T8453 - clone 7B9, purified from hybridoma cell culture
Synonym: Anti-TUBB2; Anti-Tubulin, β2
MDL Number: MFCD00145892
Product Type: Chemical
Immunoblotting Whole extract of Neuro-2a cells was separated on SDS-PAGE, blotted with 1 μg/mL Monoclonal Anti-β-Tubulin II Clone: 7B9 (Cat. No. T8453) and developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 7B9, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~50 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| western blot: 1-2 μg/mL using Neuro-2a cell extract | |
| UniProt accession no. | Q9BVA1  |
| Application: | Anti-β-Tubulin II antibody has been used in • immunoblotting immunoprecipitation • immunocytochemistry • western blotting • immunolabelling |
| Biochem/physiol Actions: | The αβ tubulins are involved in the formation of microtubules during cell division by the formation of protofilaments. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti- β-Tubulin II (mouse IgG1 isotype) is derived from the hybridoma 7B9 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment at the C-terminus of human β?tubulin II. Tubulin is the major building block of microtubules. This intracellular, cylindrical, filamentous structure is present in almost all eukaryotic cells. Tubulin is a heterodimer that consists of α-tubulin and β ?tubulin. Both subunits have a molecular mass of ~50 kDa and share considerable homology. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |