Activated Thiol–Sepharose® 4B
SIGMA/T8512 - lyophilized powder
MDL Number: MFCD00161935
Product Type: Chemical
Activated Thiol Sepharose® 4B is a mixed disulphide formed between 2,2′-dipyridyl disulphide and glutathione coupled to CNBr-activated Sepharose 4B
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T8512-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T8512-5G
| extent of labeling | 1 μmol per mL |
| form | lyophilized powder |
| matrix | Sepharose 4B |
| matrix activation | cyanogen bromide |
| matrix active group | glutathione 2-pyridyl disulfide |
| matrix attachment | N-terminal amino group |
| matrix spacer | 10 atoms (when ligands are coupled through the disulfide groups) |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| swelling | 1 g swells to 4-5 mL |
| Application: | Activated thiol Sepharose® 4B is used in protein chromatography, affinity chromatography and activated/functionalized matrices. Activated thiol Sepharose® 4B has been used to provide the first report of the isolation of aminopeptidase H from a reptile. Activated thiol Sepharose® 4B has also been used to purify and characterize a neuropeptide-inactivating peptidase. |
| Legal Information: | Sepharose is a registered trademark of Cytiva |
| Packaging: | 1, 5 g in poly bottle |
| Physical form: | Lyophilized powder stabilized with lactose and dextran |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |