Monoclonal Anti-β-Tubulin I+II antibody produced in mouse
SIGMA/T8535 - clone JDR.3B8, ascites fluid
Synonym: Anti-CDCBM5; Anti-TUBB; Anti-TUBB2
MDL Number: MFCD00145892
Product Type: Chemical
Western Blotting Whole extract of Chicken fibroblasts were separated on SDS-PAGE and probed with Monoclonal Anti-β-Tubulin I+II antibody produced in Mouse, Clone: JDR.3B8 (Cat. No. T8535). The antibody was developed using 1:500 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:1,000 antibody 2. 1:2,000 antibody 3. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | JDR.3B8, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2b |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, pig, chicken, human, rat, mouse |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| microarray: suitable | |
| western blot: 1:1,000 using a chicken fibroblast extract or a chicken brain microtubule proteins preparation | |
| UniProt accession no. | Q13885  |
| Q9H4B7 |
| Application: | Monoclonal Anti-β-Tubulin I+II antibody produced in mouse has been used in following studies: • immunoblot • immunofluorescence technique • immunohistochemistry. |
| Application: | Monoclonal Anti-β-Tubulin I+II antibody produced in mouse has been used in: • enzyme linked immunosorbent assay (ELISA) • immunohistochemistry • western blotting • immunoblotting • dot blot • immunocytochemistry |
| Biochem/physiol Actions: | β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP). Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β-tubulin and inhibits natural killer (NK) cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. Tubulin β 2B class IIb (TUBB2B)/β-tubulin II plays a vital role in migration/organization and axon guidance processes. Mutation in β-tubulin gene is associated with the development of asymmetric polymicrogyria (PMG). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-β-Tubulin Isotype I + II (mouse IgG2b isotype) is derived from the JDR.3B8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. |
| General description: | β-tubulin belongs to the subfamily of tubulins, which are the major building blocks of microtubules. β-tubulin has a molecular weight of 55kDa. β-tubulin structure is characterized with a core of two β-sheets enclosed by α-helices. In addition, it also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site and a C-terminal domain that contains the binding surface for molecular motor proteins. β-tubulin I and II is encoded by the gene mapped to human chromosome 20 and 6p25.2 respectively. |
| Immunogen: | synthetic peptide corresponding to the C-terminal sequence of human β-tubulin isotype II coupled to BSA |
| Specificity: | The antibody specifically recognizes an epitope located on isotypes I and II of β-tubulin. No cross-reactivity with other tubulin isotypes is observed. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |