Monoclonal Anti-Topoisomerase I antibody produced in mouse
SIGMA/T8573 - clone Mab1, purified immunoglobulin, buffered aqueous solution
MDL Number: MFCD03097334
Product Type: Chemical
Western Blotting Whole extract of 293T cells were separated on SDS-PAGE and probed with Monoclonal Anti-Topoisomerase I antibody produced in Mouse, Clone: Mab1 (Cat. No. T8573). The antibody was developed using 1:5,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A2304) Lanes 1. 2 μg/mL antibody 2. 1 μg/mL antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | Mab1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~100 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | microarray: suitable |
| western blot: 1-2 μg/mL using A231 human kidney cells | |
| UniProt accession no. | P11387  |
| Application: | Monoclonal Anti-Topoisomerase I antibody produced in mouse has been used in western blot analysis. |
| Biochem/physiol Actions: | DNA topoisomerases are enzymes that control the amount of supercoiling in DNA. Without topoisomerases, DNA cannot replicate normally. Type I enzymes of DNA topoisomerases introduce transient single strand breaks in DNA. Recently it was reported that DNA topoisomerase I is involved in modulation of RNA and is present in retroviral particles, like human immunodeficiency virus (HIV1). Furthermore, the enzyme enhances HIV-1 cDNA production in reverse transcription assays in vitro. Topoisomerase I (TOP1) serves as swivel at the time of replication, transcription and recombination, which helps in releasing over winding of duplex DNA. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Topoisomerase I (mouse IgG1 isotype) is derived from the Mab1 hybridoma produced by the fusion of SP2/0 mouse myeloma cells and splenocytes from BALB/c mice immunized with human purified topoisomerase I. The protein family of DNA topoisomerases is divided into two types (type I and II). The type I enzymes include eukaryotic and bacterial topoisomerase I and III. Human DNA Topoisomerase I belong to the subtype IB enzymes. The protein has four distinct domains. There are four nuclear localization signals in the N-terminal domain. A linker domain of 77 amino acids follows the core domain. The fourth domain, which is 53 amino acids long, contains the active site Tyr723. Topoisomerase I (TOP1) gene is located on human chromosome 20q12. |
| Immunogen: | purified human topoisomerase I |
| Packaging: | 200 μL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| WGK Germany | 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |