Anti-β-Tubulin III (neuronal) antibody, Mouse monoclonal
SIGMA/T8578 - ~1.0 mg/mL, clone 2G10, purified from hybridoma cell culture
Synonym: Anti-β-Tubulin Class III; Anti-Neuron-specifc class III β-tubulin; Anti-TUBB4; Anti-Tubb3; Anti-Tubulin β-4; Anti-Tubulin β-III; Anti-Tubulin, β−3
MDL Number: MFCD01323870
Product Type: Chemical
Western Blotting JOURNAL CITATION: Dynamic recruitment of the curvature-sensitive protein ArhGAP44 to nanoscale membrane deformations limits exploratory filopodia initiation in neurons. By: Galic, M., Tsai, F. C., et al. in Elife, 2014. PubMed ID: 25498153 Image collected and cropped by CiteAb from the following publication, (https://elifesciences.org/articles/03116), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Severe drug-induced repetitive behaviors and striatal overexpression of VAChT in ChAT-ChR2-EYFP BAC transgenic mice. By: Crittenden, J. R., Lacey, C. J., et al. in Front Neural Circuits, 2014. PubMed ID: 24904300 Image collected and cropped by CiteAb from the following publication, (http://journal.frontiersin.org/article/10.3389/fncir.2014.00057/abstract), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Dynamic recruitment of the curvature-sensitive protein ArhGAP44 to nanoscale membrane deformations limits exploratory filopodia initiation in neurons. By: Galic, M., Tsai, F. C., et al. in Elife, 2014. PubMed ID: 25498153 Image collected and cropped by CiteAb from the following publication, (https://elifesciences.org/articles/03116), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence SH-SY-5Y cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 2 μg/mL Monoclonal Anti-β-Tubulin III (neuronal), Clone: 2G10 (Cat. No. T8578). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Monoclonal Anti-β-Tubulin III (neuronal) Clone: 2G10 (Cat. No. T8578). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. SK-N-SH 2. SH-SY5Y 3. U87 4. COS7 5. PC12 6. Neuro-2a
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2G10, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen ~50 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 0.25-0.5 μg/mL using PC12 cell extract | |
| UniProt accession no. | Q13509  |
| Application: | Monoclonal Anti-β-Tubulin III (neuronal) antibody produced in mouse has been used in following studies: • Immunofluorescence. • Immunocytochemistry. |
| Biochem/physiol Actions: | Tubulin β 3 class III (TUBB3) plays a vital role in nervous system development and axon guidance. Mutation of the gene encoding protein leads to the ocular motility disorder, congenital fibrosis of the extraocular muscle type 3 (CFEOM3), and various neurological syndromes. Altered expression of the protein affects microtubule dynamics and microtubule-kinesin interactions. Increased expression of TUBB3 is observed in tumor tissues. Decreased expression of TUBB3 can be considered as an important marker for poor prognosis of cutaneous malignant melanoma. TUBB3 is implicated in the suppression of invasive growth of tumor tissue;therefore, it is considered to be a potential target for development of antitumor drugs. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Tubulin β 3 class III (TUBB3) also known as β-Tubulin III, is encoded by the gene mapped to human chromosome 16q24.3. TUBB3 protein expression is restricted to neurons. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |