Trichloroacetic acid
SIGMA/T8657 - suitable for electrophoresis, suitable for fixing solution (for IEF and PAGE gels), ≥99%
Synonym: TCA
CAS Number: 76-03-9
Empirical Formula (Hill Notation): C2HCl3O2
Molecular Weight: 163.39
EC Number: 200-927-2
MDL Number: MFCD00004177
Linear Formula: Cl3CCOOH
Product Type: Chemical
anion traces | chloride (Cl-): ≤0.001% |
nitrate (NO3-): ≤0.002% | |
phosphate (PO43-): ≤5 ppm | |
sulfate (SO42-): ≤0.02% | |
application(s) | cell analysis |
assay | ≥99% |
bp | 196 °C (lit.) |
cation traces | Fe: ≤0.0005% |
heavy metals (as Pb): ≤0.002% | |
density | 1.62 g/mL at 25 °C (lit.) |
form | powder or flakes |
InChI | 1S/C2HCl3O2/c3-2(4,5)1(6) |
InChI key | YNJBWRMUSHSURL-UHFFFAOYSA |
mp | 54-58 °C (lit.) |
Quality Level | 200 ![]() |
refractive index | n |
SMILES string | OC(=O)C(Cl)(Cl)Cl |
solubility | water: 1 g/mL, clear, colorless |
storage temp. | 2-8°C |
suitability | suitable for fixing solution (for IEF and PAGE gels) |
technique(s) | electrophoresis: suitable |
turbidity | ≤3 NTU (1 g/ml) |
vapor density | <1 (vs air) |
vapor pressure | 1 mmHg ( 51 °C) |
Application: | Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy. |
Application: | Trichloroacetic acid has been used: • for hydrolysis of DNA isolated from the cell • for precipitation of glutathione isolated from seminal plasma • as a fixative in microscopy |
General description: | Trichloroacetic acid (TCA) is produced during the chlorination of water comprising natural organic material. It is a novel reagent used in clinical laboratories for deproteinization of body fluids. In addition, TCA is also used in the treatment of genital warts and acne scars. |
Packaging: | 1 kg in glass bottle |
Packaging: | 250, 500 g in glass bottle |
Symbol | ![]() ![]() ![]() |
Signal word | Danger |
Hazard statements | H314 - H335 - H410 |
Precautionary statements | P260 - P271 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338 |
Hazard Codes | C,N |
Risk Statements | 35-50/53 |
Safety Statements | 26-36/37/39-45-60-61 |
RIDADR | UN 1839 8 / PGII |
WGK Germany | WGK 2 |
Flash Point(F) | 235.4 °F - closed cup |
Flash Point(C) | > 113 °C - closed cup |
Purity | ≥99% |
bp | 196 °C (lit.) |
mp | 54-58 °C (lit.) |
Density | 1.62 g/mL at 25 °C (lit.) |
Refractive Index | n |
Storage Temp. | 2-8°C |
UNSPSC | 12352200 |