3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System
SIGMA/T8665 - peroxidase substrate
Synonym: TMB
Product Type: Chemical
Sigma offers specially formulated TMB liquid substrate systems for ELISA requiring a soluble end-product and for western blotting procedures that require an insoluble end-product. TMB Liquid Substrate System for peroxidase-based ELISA procedures where the soluble blue end product can be read at 370 nm or 655 nm. The addition of an acidic stopping solution produces a yellow color that can be read at 450 nm. The Sigma TMB Substrate System for Membranes produces an insoluble, dark blue end-product, and is not recommended for ELISA or immunohistochemical applications.
| form | liquid |
| Quality Level | 300  |
| shipped in | wet ice |
| storage condition | protect from light |
| storage temp. | 2-8°C |
| Application: | TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications. 3,3′,5,5′-Tetramethylbenz |
| Caution: | This substrate is very light-sensitive and should be protected from direct sunlight or UV sources. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Physical form: | Ready-to-use. |
| Symbol |    GHS02,GHS06,GHS08 |
| Signal word | Danger |
| Hazard statements | H226 - H301 + H311 + H331 - H319 - H370 |
| Precautionary statements | P210 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P311 - P305 + P351 + P338 |
| Hazard Codes | T |
| Risk Statements | 10-23/24/25-39/23/24/25 |
| Safety Statements | 36/37-45 |
| RIDADR | UN 1993C 3 / PGIII |
| WGK Germany | WGK 2 |
| Flash Point(F) | 129.9 °F - closed cup |
| Flash Point(C) | 54.4 °C - closed cup |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |