Thioredoxin human
SIGMA/T8690 - ≥90% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), essentially salt-free, lyophilized powder, ≥5 U/mg
CAS Number: 52500-60-4
MDL Number: MFCD00132423
Product Type: Chemical
SDS-PAGE Thioredoxin human (Cat. No. T8690) was assessed on SDS-PAGE.
Unit Definition 1 unit of thioredoxin activity causes an increase of 1.0 OD650 per minute at 25C at pH 7.0 in presence of 1mM DDT. DTT-(SH)2 = DL - Dithiothreitol Insulin-S2 = Insulin (native disulfide bridge) Insulin-(SH)2 = Insulin (reduced disulfides) DTT-S2 = DL - Dithiothreitol (disulfide bridge)
| application(s) | cell analysis |
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| form | essentially salt-free, lyophilized powder |
| mol wt | 14 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli (N-terminal histidine tagged) |
| solubility | water: soluble 0.50—0.60 mg/mL, clear to slightly hazy, colorless to faint yellow or tan |
| specific activity | ≥5 U/mg |
| storage temp. | −20°C |
| suitability | suitable for molecular biology |
| technique(s) | activity assay: suitable |
| UniProt accession no. | P10599  |
| Application: | Thioredoxin human can be used for in vitro thioredoxin assay. The product can also be used for assessing Thioredoxin reductase (TRXR) enzyme activity. |
| Application: | Thioredoxin human has been used: • in mitogen-activated protein kinase kinase 4 (MKK4) in vitro kinase assay • to study its role in transcription regulation, RNA processing and nuclear pore function in human cells • in proteomics identification of Trx1 reduction target proteins from the hearts of cardiac specific transgenic (Tg)-Trx1 mice |
| Biochem/physiol Actions: | Human Thioredoxin is a recombinant, N-terminal histidine tagged protein. The product induces proliferation in lymphoid cells, fibroblasts as well as in numerous human solid tumor cell lines.The product also stimulates growth in normal as well as leukemic B cell, modifies the transcriptional activity of AP-1and inhibits the apoptosis signal-regulating kinase 1 (ASK1).Thioredoxin (TRX) plays a role in protecting the cells fromelectrophiles, a cytotoxic agent. |
| Biochem/physiol Actions: | Mediates the reduction of disulfide bonds in proteins. |
| Biochem/physiol Actions: | Thioredoxin (TXN) has disulfide-reducing activity. It acts as a cytokine to reduce inflammation. TXN functions as a scavenger for reactive oxygen intermediates (ROIs), which are produced in various oxidative stress. Thioredoxin induces cellular proliferation to decrease oxidative stress. TXN is a prognostic biomarker for various cancers, such as lung, pancreatic and hepatocellular carcinoma. It contributes to protein reduction, tissue development and redox regulation. |
| General description: | Cloned from Jurkat cell cDNA. Identical to the ADF/Trx (GenBank® Accession Number X77584) and the thioredoxin from human placenta (GenBank® Accession Number AF085844). |
| General description: | Thioredoxin (TXN) is a 12 kDa ubiquitous protein. The gene is located on human chromosome 9q31.3. TXN is mainly located in the cytoplasm.Thioredoxin contains a redox-active dithiol in the active site. Itexists in several forms, the most prevalent ones being the cytosolic (TRX1) andmitochondrial (TRX2) forms. |
| Legal Information: | GenBank is a registered trademark of United States Department of Health and Human Services |
| Other Notes: | One unit will cause a ΔA650 of 1.0 in 1 min at 25 °C in the insulin reduction assay. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: ≥5 U/mg |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |