TrkB/Fc Chimera human
SIGMA/T8694 - >90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder
MDL Number: MFCD03458837
Product Type: Chemical
assay | >90% (SDS-PAGE) |
biological source | human |
form | lyophilized powder |
impurities | endotoxin, tested |
mol wt | 120-130 kDa by SDS-PAGE (reducing) |
calculated mol wt 71 kDa | |
packaging | pkg of 100 μg |
potency | 0.1-0.4 μg/mL ED50 |
Quality Level | 200 ![]() |
recombinant | expressed in NSO cells |
storage condition | avoid repeated freeze/thaw cycles |
storage temp. | −20°C |
technique(s) | western blot: suitable |
UniProt accession no. | Q16620 ![]() ![]() |
Analysis Note: | Inhibits BDNF-induced proliferation of TrkB transfected cells, BaF-TrkB-BD. |
Application: | TrkB/Fc Chimera human can be used in synaptoneurosome preparation and western blot analysis. Intracerebroventricular administeration of TrkB/Fc chimera human blocks TrkB ligand signaling and was used to study the role of BDNF in ATN response augmentation. |
Biochem/physiol Actions: | Member of the Trk family tyrosine kinase receptors. Binds specifically to mediate neurotrophins BDNF and NT-4/5. |
Biochem/physiol Actions: | TrkB/Fc Chimera human consist of extracellular domain of human TrkB1 fused to histidine-tagged Fc region of human IgG1 by using a polypeptide linker. TrkB belongs to tyrosine kinase receptors family and facilitates the hippocampal development as well as regulating the synaptic plasticity that underlies long-term potentiation and learning. Brain derived neurotrophic factor (BDNF) and Neurotrophin 4 or 5 are preferred ligands for TrkB. Nevertheless it can also bind to NTF3/neurotrophin-3 that is less efficient in activating the receptor but regulates neuron survival through NTRK2. |
Other Notes: | Extracellular domain of human TrkB fused to the C-terminal histidine-tagged Fc region of human IgG1. |
Physical form: | Lyophilized from 0.2 μm filtered solution in phosphate buffered saline. |
Reconstitution: | Reconstitute to 100 μg/mL in phosphate buffered saline. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | >90% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 51111800 |