TrkB/Fc Chimera human
SIGMA/T8694 - >90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder
MDL Number: MFCD03458837
Product Type: Chemical
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| assay | >90% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | 120-130 kDa by SDS-PAGE (reducing) |
| calculated mol wt 71 kDa | |
| packaging | pkg of 100 μg |
| potency | 0.1-0.4 μg/mL ED50 |
| Quality Level | 200  |
| recombinant | expressed in NSO cells |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | western blot: suitable |
| UniProt accession no. | Q16620  |
| Analysis Note: | Inhibits BDNF-induced proliferation of TrkB transfected cells, BaF-TrkB-BD. |
| Application: | TrkB/Fc Chimera human can be used in synaptoneurosome preparation and western blot analysis. Intracerebroventricular administeration of TrkB/Fc chimera human blocks TrkB ligand signaling and was used to study the role of BDNF in ATN response augmentation. |
| Biochem/physiol Actions: | Binds specifically to mediate neurotrophins BDNF and NT-4/5. |
| Biochem/physiol Actions: | Member of the Trk family tyrosine kinase receptors. Binds specifically to mediate neurotrophins BDNF and NT-4/5. |
| Biochem/physiol Actions: | TrkB/Fc Chimera human consist of extracellular domain of human TrkB1 fused to histidine-tagged Fc region of human IgG1 by using a polypeptide linker. TrkB belongs to tyrosine kinase receptors family and facilitates the hippocampal development as well as regulating the synaptic plasticity that underlies long-term potentiation and learning. Brain derived neurotrophic factor (BDNF) and Neurotrophin 4 or 5 are preferred ligands for TrkB. Nevertheless it can also bind to NTF3/neurotrophin-3 that is less efficient in activating the receptor but regulates neuron survival through NTRK2. |
| Other Notes: | Extracellular domain of human TrkB fused to the C-terminal histidine-tagged Fc region of human IgG1. |
| Physical form: | Lyophilized from 0.2 μm filtered solution in phosphate buffered saline. |
| Preparation Note: | Reconstitute to 100 μg/mL in phosphate buffered saline. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >90% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |