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3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate

SIGMA/T8768 - powder, ≥98%

Synonym: TMB dihydrochloride

CAS Number: 207738-08-7
Empirical Formula (Hill Notation): C16H20N2 · 2HCl · xH2O
Molecular Weight: 313.27 (anhydrous basis)
MDL Number: MFCD00150104
Linear Formula: [-C6H2(CH3)2-4-NH2]2·2HCl·xH2O
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-T8768-100MG 100 mg
 $82.80
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45-T8768-1G 1 g
 $129.00
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45-T8768-5G 5 g
 $440.00
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Sigma offers specially formulated TMB liquid substrate systems for ELISA requiring a soluble end-product and for western blotting procedures that require an insoluble end-product. TMB Liquid Substrate System for peroxidase-based ELISA procedures where the soluble blue end product can be read at 370 nm or 655 nm. The addition of an acidic stopping solution produces a yellow color that can be read at 450 nm. The Sigma TMB Substrate System for Membranes produces an insoluble, dark blue end-product, and is not recommended for ELISA or immunohistochemical applications.

 

assay ≥98%
form powder
InChI 1S/C16H20N2.2ClH.H2O/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;;/h5-8H,17-18H2,1-4H3;2*1H;1H2
InChI key KVCWTKDFVVSVSJ-UHFFFAOYSA-N
Quality Level 200 
SMILES string O.Cl.Cl.Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
solubility acetic acid: water (1:2): 20 mg/mL, clear to slightly hazy
storage temp. 2-8°C
Application: 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate has also been used as a horseradish peroxidase substrate in enzyme linked immunosorbent assay and reverse dot blot (RDB).
Application: Noncarcinogenic substitute for benzidine.
General description: 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase. It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥98%
Storage Temp. 2-8°C
UNSPSC 12352204

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