3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate
SIGMA/T8768 - powder, ≥98%
Synonym: TMB dihydrochloride
CAS Number: 207738-08-7
Empirical Formula (Hill Notation): C16H20N2 · 2HCl · xH2O
Molecular Weight: 313.27 (anhydrous basis)
MDL Number: MFCD00150104
Linear Formula: [-C6H2(CH3)2-4-NH2]2·2HCl·xH2O
Product Type: Chemical
Sigma offers specially formulated TMB liquid substrate systems for ELISA requiring a soluble end-product and for western blotting procedures that require an insoluble end-product. TMB Liquid Substrate System for peroxidase-based ELISA procedures where the soluble blue end product can be read at 370 nm or 655 nm. The addition of an acidic stopping solution produces a yellow color that can be read at 450 nm. The Sigma TMB Substrate System for Membranes produces an insoluble, dark blue end-product, and is not recommended for ELISA or immunohistochemical applications.
| assay | ≥98% |
| form | powder |
| InChI | 1S/C16H20N2.2ClH.H2O/c1-9 |
| InChI key | KVCWTKDFVVSVSJ-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | O.Cl.Cl.Cc1cc(cc(C)c1N)-c |
| solubility | acetic acid: water (1:2): 20 mg/mL, clear to slightly hazy |
| storage temp. | 2-8°C |
| Application: | 3,3′,5,5′-Tetramethylbenz |
| Application: | Noncarcinogenic substitute for benzidine. |
| General description: | 3,3′,5,5′-Tetramethylbenz |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |