Trehalase from porcine kidney
SIGMA/T8778 - buffered aqueous glycerol solution, ≥1.0 units/mg protein
Synonym: α,α-Trehalose glucohydrolase
CAS Number: 9025-52-9
MDL Number: MFCD00132462
Product Type: Chemical
| concentration | 0.5-10.0 mg/mL protein (BCA) |
| form | buffered aqueous glycerol solution |
| Quality Level | 200  |
| specific activity | ≥1.0 units/mg protein |
| storage temp. | −20°C |
| Application: | Trehalase has been used: • in a study to assess changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves • in a study to investigate growth arrest by trehalose-6-phosphate • to quantify the intracellular trehalose in Lactobacillus casei cells |
| Biochem/physiol Actions: | Trehalase catalyzes the hydrolysis of trehalose to produce two glucose units. It mediates flight metabolism, molting induced chitin production and tolerance towards cold temperature. |
| General description: | Trehalase is a glycosidase that occurs in many forms and is found in all tissues. It belongs to the glycoside hydrolase 37 (GH37) family of the carbohydrate-active enzyme (CAZy) classification. |
| General description: | Trehalose is the main haemolymph sugar in most insects and is potentially a prime target for an invading pathogenic fungus. |
| Legal Information: | Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow |
| Physical form: | Solution in 50% glycerol containing 1% Triton™ X-100 and 25 mM potassium phosphate, pH 6.5 |
| Unit Definition: | One unit will convert 1.0 μmole of trehalose to 2.0 μmoles of glucose per min at pH 5.7 at 37 °C (liberated glucose determined at pH 7.5). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥1.0 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.28 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |