Triton^™ X-100

SIGMA/T8787 - for molecular biology

Synonym: t-Octylphenoxypolyethoxyethanol; Polyethylene glycol tert-octylphenyl ether

CAS Number: 9036-19-5
MDL Number: MFCD00128254
Linear Formula: t-Oct-C₆H₄-(OCH₂CH₂)_xOH, x= 9-10
Product Type: Chemical

aggregation number	100-155
application(s)	diagnostic assay manufacturing hematology histology
CMC	0.2-0.9 mM (20-25°C)
density	1.06 g/mL at 25 °C (lit.)
description	non-ionic
foreign activity	DNase and RNase, none detected
form	liquid
grade	for molecular biology
HLB	13.5
InChI	1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
InChI key	HNLXNOZHXNSSPN-UHFFFAOYSA-N
mol wt	average mol wt 625
	micellar avg mol wt 80,000
mp	6 °C
pH	9.7
Quality Level	200
SMILES string	CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
solubility	water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
storage temp.	room temp
technique(s)	protein purification: suitable
	protein quantification: suitable
	western blot: suitable
transition temp	cloud point 65 °C
	pour point ~7 °C

Application:	Triton^™ X-100 has been used: • In immunohistochemistry for staining the Flat-mount retinas • Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay • To permeabilise cells during Immunofluorescent microscopic studies • As a positive control in LDH assay to determine the cell membrane integrity • For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure • For the preparation of outer membrane protein exctract • As a component of extraction buffer along with tris-HCl, NaCl, CaCl₂, ZnCl₂, Brij 35 for homogenization of mice lung cells • In the treatment of tissue sections for Immunofluorescence labeling
Biochem/physiol Actions:	Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.
General description:	Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.
Legal Information:	Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Packaging:	50, 100, 250 mL in poly bottle