Triton™ X-100
SIGMA/T8787 - Molecular Biology
Synonym: t-
CAS Number: 9036-19-5
Empirical Formula (Hill Notation): (C2H4O)nC14H22O
MDL Number: MFCD00128254
Linear Formula: t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
Product Type: Chemical
| aggregation number | 100-155 |
| application(s) | hematology histology |
| biological source | synthetic (organic) |
| cation traces | Fe: <5 ppm |
| heavy metals: <5 ppm | |
| K: <0.05% | |
| Na: <0.1% | |
| CMC | 0.2-0.9 mM (20-25°C) |
| density | 1.06 g/mL at 25 °C (lit.) |
| description | non-ionic |
| foreign activity | DNase and RNase, none detected |
| form | liquid |
| grade | Molecular Biology |
| HLB | 13.5 |
| impurities | <1.00% water (Karl Fischer) |
| InChI | 1S/C28H50O8/c1-27(2,3)24- |
| InChI key | HNLXNOZHXNSSPN-UHFFFAOYSA |
| mol wt | average mol wt 625 |
| micellar avg mol wt 80,000 | |
| mp | 6 °C |
| pH | 9.7 |
| Quality Level | 200  |
| SMILES string | CC(C)(C)CC(C)(C)c1ccc(OCC |
| solubility | water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow |
| storage temp. | room temp |
| technique(s) | protein purification: suitable |
| protein quantification: suitable | |
| western blot: suitable | |
| transition temp | cloud point 65 °C |
| pour point ~7 °C |
| Application: | Triton™ X-100 has been used: • In immunohistochemistry for staining the Flat-mount retinas • Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay • To permeabilise cells during Immunofluorescent microscopic studies • As a positive control in LDH assay to determine the cell membrane integrity • For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure • For the preparation of outer membrane protein exctract • As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells • In the treatment of tissue sections for Immunofluorescence labeling |
| Biochem/physiol Actions: | Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions. |
| Features and Benefits: | • Ideal for sensitive molecular biology research applications • Tested for DNase and RNase • Non-ionic nondenaturing detergent • Superior wetting agent and emulsifier • Highly versatile surfactant |
| General description: | Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection. |
| Legal Information: | Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Packaging: | 50, 100, 250 mL in poly bottle |
| Symbol |    GHS05,GHS07,GHS09 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H318 - H410 |
| Precautionary statements | P264 - P273 - P280 - P280 - P305 + P351 + P338 + P310 - P391 |
| Hazard Codes | Xn,N |
| Risk Statements | 22-36-51/53 |
| Safety Statements | 26-39-61 |
| RIDADR | UN 3082 9 / PGIII |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 6 °C |
| Density | 1.06 g/mL at 25 °C (lit.) |
| Storage Temp. | room temp |
| UNSPSC | 12161900 |