Triton™ X-100
SIGMA/T8787 - for molecular biology
Synonym: t-
CAS Number: 9036-19-5
MDL Number: MFCD00128254
Linear Formula: t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
Product Type: Chemical
aggregation number | 100-155 |
application(s) | diagnostic assay manufacturing hematology histology |
CMC | 0.2-0.9 mM (20-25°C) |
density | 1.06 g/mL at 25 °C (lit.) |
description | non-ionic |
foreign activity | DNase and RNase, none detected |
form | liquid |
grade | for molecular biology |
HLB | 13.5 |
InChI | 1S/C28H50O8/c1-27(2,3)24- |
InChI key | HNLXNOZHXNSSPN-UHFFFAOYSA |
mol wt | average mol wt 625 |
micellar avg mol wt 80,000 | |
mp | 6 °C |
pH | 9.7 |
Quality Level | 200 ![]() |
SMILES string | CC(C)(C)CC(C)(C)c1ccc(OCC |
solubility | water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow |
storage temp. | room temp |
technique(s) | protein purification: suitable |
protein quantification: suitable | |
western blot: suitable | |
transition temp | cloud point 65 °C |
pour point ~7 °C |
Application: | Triton™ X-100 has been used: • In immunohistochemistry for staining the Flat-mount retinas • Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay • To permeabilise cells during Immunofluorescent microscopic studies • As a positive control in LDH assay to determine the cell membrane integrity • For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure • For the preparation of outer membrane protein exctract • As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells • In the treatment of tissue sections for Immunofluorescence labeling |
Biochem/physiol Actions: | Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions. |
General description: | Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection. |
Legal Information: | Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow |
Packaging: | 50, 100, 250 mL in poly bottle |
Symbol | ![]() ![]() ![]() |
Signal word | Danger |
Hazard statements | H302 - H315 - H318 - H410 |
Precautionary statements | P264 - P273 - P280 - P280 - P305 + P351 + P338 + P310 - P391 |
Hazard Codes | Xn,N |
Risk Statements | 22-36-51/53 |
Safety Statements | 26-39-61 |
RIDADR | UN 3082 9 / PGIII |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
mp | 6 °C |
Density | 1.06 g/mL at 25 °C (lit.) |
Storage Temp. | room temp |
UNSPSC | 12161900 |