Monoclonal Anti-T7 tag antibody produced in mouse
SIGMA/T8823 - purified immunoglobulin, buffered aqueous solution
Synonym: T7 Tag antibody
MDL Number: MFCD02689159
Product Type: Chemical
Western Blotting Recombinant T7-tagged β-galactosidase were separated on SDS-PAGE and probed with Anti-T7 tag antibody, Mouse monoclonal, Clone: T7 Tag (Cat. No. T8823) followed by Anti Mouse IgG (Fab)-Peroxidase Lanes 1. 10 μg/mL antibody 2. 2 μg/mL antibody 3. 1 μg/mL antibody 4. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | T7 Tag |
| concentration | ~2 mg/mL |
| form | buffered aqueous solution |
| isotype | IgM |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| technique(s) | western blot (chemiluminescent): 1-2 μg/mL using recombinant T7-tagged β-galactosidase |
| Analysis Note: | Antibody is suitable for ELISA and immunoblotting. |
| Application: | Monoclonal Anti-T7 tag antibody produced in mouse has been used in western blotting. |
| General description: | The Mouse monoclonal Anti-T7 tag antibody recognizes the T7 tag sequence on T7-tagged fusion proteins. The T7 tag functions as a marker in many expression vectors. |
| General description: | The T7 tag is a 11 amino acid peptide, encoded in the leader sequence of T7 bacteriophage gene 10. This gene encodes a T7 major capsid protein. |
| Immunogen: | synthetic peptide corresponding to the T7-tag (MASMTGGQQMG-K), conjugated to KLH |
| Physical form: | Solution at approximately 2 mg/ml in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |