Thrombin from human plasma
SIGMA/T8885 - lyophilized powder, Suitable for routine use in the thrombin time test
Synonym: Factor IIa
CAS Number: 9002-04-4
EC Number: 232-648-7
MDL Number: MFCD00082072
Product Type: Chemical
biological source | human plasma |
form | lyophilized powder |
impurities | HIV, hepatitis B and hepatitis C, tested negative |
packaging | vial of ≥10 NIH units |
Quality Level | 300 ![]() |
storage temp. | −20°C |
UniProt accession no. | P00734 ![]() ![]() |
Analysis Note: | The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations. |
Application: | Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study that compared efficacy and safety of low-molecular-weight heparins (LMWHs) to other anticoagulants as venous thromboembolism prophylaxis in major orthopedic surgery. |
Biochem/physiol Actions: | Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B. |
General description: | Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. |
Other Notes: | View more information on thrombin ![]() ![]() |
Reconstitution: | When reconstituted with 1 ml H2O, one vial will contain 10 mg bovine serum albumin, 0.15 M NaCl and 0.05 M sodium citrate, pH 6.5 |
Unit Definition: | Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard |
Symbol | ![]() ![]() |
Signal word | Danger |
Hazard statements | H315 - H319 - H334 - H335 |
Precautionary statements | P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
Hazard Codes | Xn |
Risk Statements | 36/37/38-42 |
Safety Statements | 22-24-26-36/37 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
Enzyme Commission (EC) Number | 3.4.21.5 ![]() ![]() ![]() ![]() ![]() |
UNSPSC | 12352204 |