Topoisomerase II α human
SIGMA/T8944 - (Single band on SDS-PAGE), liquid
Synonym: Topoisomerase II alpha; TOPO® II, p170
MDL Number: MFCD03458766
Product Type: Chemical
| form | liquid |
| mol wt | 170 kDa |
| Quality Level | 200  |
| storage temp. | −70°C |
| UniProt accession no. | P11388  |
| Application: | Human topoisomerase II α has been used in DNA cleavage assay. |
| Application: | Topoisomerase II α has been used in a study to assess aging processes in the human brain. Topoisomerase II α has also been used in a study to investigate its activity in HIV-1 replication. |
| Biochem/physiol Actions: | Used to study DNA structure and topology. |
| General description: | Topoisomerase II α (TopoIIα) is a gene product with conserved catalytic activities and it promotes the progression of DNA damage. The α isoform is present in proliferating cells. |
| General description: | Topoisomerase II α is encoded by TOP2A gene, which is located on 17q21.2 in the human chromosome. It is essential for controlling the topological state of DNA by reversibly breaking the DNA strands during replication, recombination and transcription. TOP2A expression increases at late S phase and during G2/M of the cell cycle and decreases after mitosis. Polymorphism with TOP2A causes chromosome instability and tumorigenesis. TOP2A causes tumor proliferation and invasiveness in various cancer types like breast cancer, ovarian cancer and prostate cancer. |
| Legal Information: | TOPO is a registered trademark of Life Technologies |
| Physical form: | Buffered aqueous glycerol solution, containing Trizma buffer (pH 7.7). Other components are present in the solution, and lot-specific information on these components may be obtained from Technical Service. |
| Preparation Note: | Recombinant enzyme overexpressed in a proprietary eukaryotic cell line. |
| Unit Definition: | One unit will decatenate 0.2 μg of kinetoplast DNA in 30 minutes at 37°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| Enzyme Commission (EC) Number | 5.99.1.3 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |