Trypsin inhibitor from Glycine max (soybean)

SIGMA/T9003 - lyophilized powder

Synonym: SBTI

CAS Number: 9035-81-8
EC Number: 232-906-9
MDL Number: MFCD00082103
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-T9003-25MG	25 mg	$76.90 1/EA	Add To Favorites
45-T9003-100MG	100 mg	$209.00 1/EA	Add To Favorites
45-T9003-250MG	250 mg	$337.00 1/EA	Add To Favorites
45-T9003-500MG	500 mg	$731.00 1/EA	Add To Favorites
45-T9003-1G	1 g	$1080.00 1/EA	Add To Favorites
45-T9003-5G	5 g	$3580.00 1/EA	Add To Favorites

Trypsin Inhibitor (Kunitz) (soybean)

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T9003-5G

Properties
Descriptions
Safety Info.
Basic Data

application(s)	diagnostic assay manufacturing
biological source	Glycine max (soybean)
form	lyophilized powder
mol wt	20.1 kDa
Quality Level	300
solubility	H₂O: >10 mg/mL
	phosphate buffer: >10 mg/mL (hazy above 10 mg/ml)
	soluble
storage temp.	2-8°C

Analysis Note:	One mg of trypsin inhibitor will inhibit 1.0-3.0 mg trypsin of activity ~10,000 BAEE units per mg protein.
Application:	Trypsin has been used in a study to assess the potential application in animal cell culture of an alkaline protease from a non-toxigenic mangrove isolate of Vibrio sp. V26. Trypsin has also been used in a study to improve the detection of fungi in eosinophilic mucin.
Biochem/physiol Actions:	This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.
Other Notes:	One trypsin unit = A₂₅₃ of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.
Other Notes:	One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.
Other Notes:	The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges.
Packaging:	1, 5 g in glass bottle
Packaging:	100, 250, 500 mg in glass bottle
Packaging:	25 mg in poly bottle
Preparation Note:	The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet. Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.

Symbol	GHS08
Signal word	Danger
Hazard statements	H317 - H334
Precautionary statements	P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
Hazard Codes	Xn
Risk Statements	42/43
Safety Statements	22-36/37-45
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3

Storage Temp.	2-8°C
UNSPSC	12352204

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