Trypsin inhibitor from Glycine max (soybean)
SIGMA/T9003 - lyophilized powder
Synonym: SBTI
CAS Number: 9035-81-8
EC Number: 232-906-9
MDL Number: MFCD00082103
Product Type: Chemical
Trypsin Inhibitor (Kunitz) (soybean)
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T9003-5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T9003-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T9003-250MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T9003-500MG
| application(s) | diagnostic assay manufacturing |
| biological source | Glycine max (soybean) |
| form | lyophilized powder |
| mol wt | 20.1 kDa |
| Quality Level | 300  |
| solubility | H2O: >10 mg/mL |
| phosphate buffer: >10 mg/mL (hazy above 10 mg/ml) | |
| soluble | |
| storage temp. | 2-8°C |
| Analysis Note: | One mg of trypsin inhibitor will inhibit 1.0-3.0 mg trypsin of activity ~10,000 BAEE units per mg protein. |
| Application: | Trypsin has been used in a study to assess the potential application in animal cell culture of an alkaline protease from a non-toxigenic mangrove isolate of Vibrio sp. V26. Trypsin has also been used in a study to improve the detection of fungi in eosinophilic mucin. |
| Biochem/physiol Actions: | This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent. |
| Components: | The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges. |
| Packaging: | 1, 5 g in glass bottle |
| Packaging: | 100, 250, 500 mg in glass bottle |
| Packaging: | 25 mg in poly bottle |
| Preparation Note: | The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet. Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C. |
| Unit Definition: | One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginin |
| Unit Definition: | One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 22-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |