Trypsinogen, PMSF treated from bovine pancreas
SIGMA/T9011 - For use as a marker in SDS-PAGE
CAS Number: 9002-08-8
Product Type: Chemical
| application(s) | detection |
| biological source | bovine pancreas |
| form | powder |
| mol wt | ~24 kDa |
| packaging | vial of 25 mg |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Application: | Trypsinogen, PMSF treated from bovine pancreas has been used as a non-phosphorylated control to analyze the phospho-protein α-casein from bovine milk by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). It has also been used as a molecular weight marker standard in SDS-PAGE. |
| Biochem/physiol Actions: | Trypsinogen is a pancreatic proteinase and exists as isoenzymes 1 and 2. It is an inactive precursor of trypsin and gets activated by the enteropeptidase found on the brush border membrane of enterocytes in the duodenum. It is also known to proteolytically activate itself. Trypsinogen activation is associated with acute pancreatitis as it causes pancreatic enzyme cascade which activates the remaining zymogens leading to autodigestion of the neighboring tissue. Hereditary pancreatitis was shown to be caused by a Arg-His substitution at residue 117 of trypsinogen causing auto-activation of trypsinogen to trypsin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |