Monoclonal Anti-α-Tubulin antibody produced in mouse
SIGMA/T9026 - clone DM1A, ascites fluid
Synonym: Monoclonal Anti-α-Tubulin
Product Type: Chemical
Western Blotting JOURNAL CITATION: Deletion of the alpha-synuclein locus in a subpopulation of C57BL/6J inbred mice. By: Specht, C. G. & Schoepfer, R. in BMC Neurosci, 2001. PubMed ID: 11591219 Image collected and cropped by CiteAb from this publication, provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Confocal image of the mouth of the Cryptocaryon irritans, a parasite of marine fishes, (theront stage) stained with Monoclonal Anti-α-Tubulin, clone no. DM 1A (Cat. No. T9026) and Anti-Mouse IgG-lissamine-rhodamine conjugate. Note the large bundles of microtubules which form a well-developed cytophyrigeal basket. From M. Keskintepe,Univ. of Georgia, Center for Adv. Ultrastructure Research, Athens, GA.
Immunofluorescence Chinese hamster embryonic fibroblasts in anaphase of mitosis were stained using Monoclonal Anti-α-Tubulin, clone no. DM1A (Cat. No. T9026) with Anti-Mouse-Alexa 488 (green) and DAPI (blue). From Thomas Durcan, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN.
Enhanced Validation-By Independent Antibodies HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton. Fixed cells were stained with 1:500 Monoclonal Anti-α-Tubulin antibody produced in Mouse, Clone: DM1A (Cat. No. T9026) (A) and developed using Goat Anti-Mouse IgG, Cy3 conjugate, and 1:500 Anti-α-Tubulin antibody, Mouse monoclonal, Clone: AA13 (Cat. No. T8203) (B). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate antibody. Results Two Anti-α-Tubulin antibodies, T9026 (A) And T8203 (B) target different regions of α-Tubulin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunofluorescence
Human skin fibroblasts stained with Monoclonal Anti-α-Tubulin, clone No. DM1A (Cat. No. T9026).
From Dr. J. V. Small, Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg, Austria.
Human skin fibroblasts stained with Monoclonal Anti-α-Tubulin, clone No. DM1A (Cat. No. T9026).
From Dr. J. V. Small, Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg, Austria.
Enhanced Validation-By Independent Antibodies Chicken fibroblasts cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:1,000 Monoclonal Anti-α-Tubulin antibody produced in Mouse, Clone: DM1A (Cat. No. T9026) (A). The antibody was developed using 1:40 Goat Anti-Mouse IgG (Fab specific)-FITC antibody (Cat. No. F5262), and 1 μg/mL Monoclonal Anti-α-Tubulin antibody produced in Mouse, Clone: B-5-1-2 (Cat. No. T5168) (B). The antibody was developed using 1:40 Goat Anti-Mouse IgG (Fab specific)-FITC antibody (Cat. No. F5262). Results Two Anti-α-Tubulin antibodies, T9026 (A) and T5168 (B) target different regions of α-Tubulin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human heart sections with 1:500 Monoclonal Anti-α-Tubulin, Clone: DM1A (Cat. No. T9026) using Biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence CFB cells cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with1:500 Monoclonal Anti-α-Tubulin antibody produced in mouse clone No. DM1A (Cat. No. T9026). The antibody was developed using Anti-Mouse IgG (Fab specific)-FITC antibody produced in goat (Cat. No. F5262). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:500 Monoclonal Anti-α-Tubulin Clone: DM1A (Cat. No. T9026). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa; 2. JURKAT; 3. COS7; 4. NIH-3T3; 5. PC-12; 6. RAT2; 7. CHO; 8. MDBK; 9. MDCK
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | DM1A, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| enhanced validation | independent ( antibodies) Learn more about Antibody Enhanced Validation  |
| isotype | IgG1 |
| mol wt | antigen ~50 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | yeast, mouse, amphibian, human, rat, chicken, fungi, bovine |
| storage temp. | −20°C |
| technique(s) | indirect immunofluorescence: 1:500 using cultured chicken fibroblasts |
| western blot: 1:500 using human or chicken fibroblasts | |
| UniProt accession no. | P68366  |
| Application: | Monoclonal Anti-α-Tubulin antibody produced in mouse has been used in western blot analysis and protein expression analysis. Monoclonal Anti-α-Tubulin antibody produced in mouse has been used in the detection of α-Tubulin: • in human osteosarcoma and in breast cancer cell lines by western blotting • in HeLa cells by immunofluorescence microscopy • by immunostaining • immunohistochemical detection in Xenopus embryos |
| Biochem/physiol Actions: | Tubulin α 4a (TUBA4A) mutation is associated with amyotrophic lateral sclerosis (ALS). α/β-Tubulin is the major building block of microtubules. These intracellular, hollow, cylindrical, filamentous structures are present in virtually all eukaryotic cells. Self-assembly of α/β-tubulin leads to polar microtubular structures built from linearly arranged strings of alternatively α- and β-tubulin pairs pointing in the same direction. Microtubules function as structural and mobile elements in mitosis, intracellular transport, ciliary flagellar motility and generation and maintenance of cell shape. α/β-Tubulin and γ-tubulin are members of the tubulin superfamily of proteins. α/β-Tubulin is a heterodimer which consists of one α-tubulin chain and one β-tubulin chain; each subunit has a molecular weight of 55 kDa and they share considerable homolog. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | α-tubulin, also called tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α-tubulin family, and contains 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000. Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the DM1A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Purified chick brain microtubules were used as immunogen. The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents, Product Number ISO2. The product is Protein A purified Monoclonal Anti-α-Tubulin antibody conjugated to fluorescein isothiocyanate, isomer I. It is purified by gel filtration and contains no detectable free FITC. Anti-α-Tubulin FITC antibody, Mouse monoclonal specifically recognizes an epitope in the carboxy terminal part of α-tubulin. It localizes α-tubulin in human, monkey, bovine, chicken, goat, murine, rat, gerbil, hamster, rat kangaroo, amphibia, sea urchin, trypanosome, yeast, fungi and tobacco. |
| Immunogen: | microtubules from chicken embryo brain. |
| Specificity: | The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts. |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |