Monoclonal Anti-Tubulin, Tyrosine antibody produced in mouse
SIGMA/T9028 - clone TUB-1A2, ascites fluid
Synonym: Anti-CDCBM6; Anti-CSCSC1; Anti-M40; Anti-OK/SW-cl.56; Anti-TUBB1; Anti-TUBB5
MDL Number: MFCD00164753
Product Type: Chemical
Western Blotting JOURNAL CITATION: Cadmium affects microtubule organization and post-translational modifications of tubulin in seedlings of soybean (Glycine max L.). By: Gzyl, J., Chmielowska-Bąk, J., et al. in Front Plant Sci, 2015. PubMed ID: 26594217 Image collected and cropped by CiteAb from the following publication, (http://journal.frontiersin.org/Article/10.3389/fpls.2015.00937/abstract), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry
Staining of Sertoli cell microtubules within a Stage VII seminiferous tubule of an adult male rat using Monoclonal Anti-Tyrosine Tubulin, Clone No. TUB-1A2 (Cat. No. T9028). The heavy staining surrounding the Sertoli cell nuclei indicates a collapse of the vimentin intermediate filament network following treatment. Interstitial cells are also stained.
From J. Wenz and M. Nakai, Univ. of Illinois, College of Med., Dept. of Veterinary Biosciences, Urbana, IL.
Staining of Sertoli cell microtubules within a Stage VII seminiferous tubule of an adult male rat using Monoclonal Anti-Tyrosine Tubulin, Clone No. TUB-1A2 (Cat. No. T9028). The heavy staining surrounding the Sertoli cell nuclei indicates a collapse of the vimentin intermediate filament network following treatment. Interstitial cells are also stained.
From J. Wenz and M. Nakai, Univ. of Illinois, College of Med., Dept. of Veterinary Biosciences, Urbana, IL.
Immunofluorescence Chicken fibroblasts cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:800 Monoclonal Anti-Tubulin, Tyrosine antibody produced in Mouse, Clone: TUB-1A2 (Cat. No. T9028). The antibody was developed using Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | TUB-1A2, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG3 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, plant, animal |
| storage temp. | −20°C |
| technique(s) | indirect immunofluorescence: 1:800 using cultured chicken fibroblasts |
| microarray: suitable | |
| western blot: suitable | |
| UniProt accession no. | P07437  |
| Application: | Monoclonal Anti-Tyrosine Tubulin has been used in: • indirect immunofluorescent labelling • immunoblotting technique • immunocytochemical staining |
| Biochem/physiol Actions: | Tubulin acts as a major building block of microtubules. Tubulin tyrosinylation is involved in the assembly status of tubulin. A specific tubulinyl tyrosine carboxypeptidase removes the terminal tyrosine to yield an α-tubulin terminating in a glutamic acid residue while another enzyme modifies the α-tubulin by addition of tyrosine to the carboxy terminus to offer a potential cycle of tyrosine addition and loss. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Tyrosine Tubulin (mouse IgG3 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin as a cylindrical filamentous structure and is present in almost all eukaryotic cells. Tubulin is a heterodimer which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. |
| General description: | The intracellular cylindrical filamentous structure, microtubules is mainly made up of tubulin and is present in all eukaryotic cells. Monoclonal Anti-Tyrosine antibody can be used in immunocytochemical localization of tyrosinated α tubulin by indirect immunofluorescence labelling. Monoclonal Anti-Tyrosine antibody reacts specifically with tyrosine tubulin of bovine brain, African Green Monkey kidney cells (Vero), dog kidney (MDCK), marsupial kidney (Potoroo, PtK2), mouse pituitary tumour (AtT-20), yeast, and Xenopus. |
| Immunogen: | peptide containing the carboxy-terminal amino acids of α-tubulin |
| Specificity: | The antibody reacts against tubulin′s C-terminal tyrosine in immunoblotting assays and may be used for localization of this epitope in cultured cells or tissue sections. |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |