Anti-Titin antibody, Mouse monoclonal
SIGMA/T9030 - clone T11, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Titin antibody produced in mouse; Anti-CMD1G; Anti-CMH9; Anti-CMPD4; Anti-EOMFC; Anti-HMERF; Anti-LGMD2J; Anti-LGMDR10; Anti-MYLK5; Anti-SALMY; Anti-TMD
MDL Number: MFCD00164750
Product Type: Chemical
Immunohistochemistry Frozen sections, acetone fixed Chicken cardiac muscle were stained with 1:1,000 Monoclonal Anti-Titin antibody produced in Mouse, Clone: T11 (Cat. No. T9030), followed by 1:30 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | T11, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2b |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | vertebrates, chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | electron microscopy: suitable |
| immunohistochemistry (frozen sections): suitable | |
| indirect immunofluorescence: 1:1,000 using frozen tissue sections of animal skeletal muscle | |
| western blot: suitable |
| Application: | Monoclonal Anti-Titin antibody produced in mouse has been used in following studies. • western blotting • antibody staining • immunofluorescence microscopy • immunohistochemistry |
| Biochem/physiol Actions: | Monoclonal Anti-Titin can be used for study of the elastic filaments within sarcomeric structures. It is also useful as a differentiation marker in the separation of rhabdomyosarcomas from other muscle tumors. |
| Biochem/physiol Actions: | The antibody localizes titin (connectin) in skeletal and heart muscle of a wide variety of species from cold-blooded vertebrates to human. The antibody does not cross-react with nebulin. Likewise, it does not react with smooth muscle, non-muscle tissues, or cultured cells. |
| Biochem/physiol Actions: | Titin (TTN) helps in maintaining sarcomeric structural integrity by interacting with many other myofibrillar and cytoskeletal proteins. Additionally, it is also involved in developing the passive elastic force in muscle. Genetic variations in the gene has been associated with the development of dilated cardiomyopathy (DCM). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Titin (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is a flexible, filamentous constituent of striated muscle that is thought to give rise to an elastic filament component underlying the myofibrillar organization. |
| General description: | Titin (TTN), also known as connectin, is encoded by the gene mapped to human chromosome 2q31.2. TTN is a giant elastic protein expressed in striated and smooth muscles of vertebrates. The encoded protein contains up to 300 immunoglobulin-like and fibronectin type-3-like (FN3) domains. |
| Immunogen: | titin/nebulin fraction from chicken breast muscle. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |