Tris Buffered Saline, with Tween™ 20, pH 8.0
SIGMA/T9039 - powder
Synonym: TBS; TBST
Product Type: Chemical
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| color | white |
| form | powder |
| Quality Level | 200  |
| Application: | Tris Buffered Saline, with Tween® 20, pH 8.0 has been used as a wash buffer in western blotting and enzyme linked immunosorbent assay. It has also been used along with 5% milk to block proteins on nitrocellulose in western blotting. |
| Biochem/physiol Actions: | Tris Buffered Saline with Tween 20 is predominantly used as a wash buffer and also in blocking steps of immunoblotting technique. |
| Legal Information: | TWEEN is a registered trademark of Croda International PLC |
| Packaging: | Foil pouches |
| Preparation Note: | Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.05 M Tris buffered saline (NaCl - 0.138 M; KCl - 0.0027 M); TWEEN® 20 - 0.05%, pH 8.0, at 25 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41105319 |