Tris Buffered Saline, with Tween® 20, pH 8.0
SIGMA/T9039 - powder
Synonym: TBST
Product Type: Chemical
form | powder |
Quality Level | 200 ![]() |
Application: | Tris Buffered Saline, with Tween® 20, pH 8.0 has been used as a wash buffer in western blotting and enzyme linked immunosorbent assay. It has also been used along with 5% milk to block proteins on nitrocellulose in western blotting. |
Biochem/physiol Actions: | Tris Buffered Saline with Tween 20 is predominantly used as a wash buffer and also in blocking steps of immunoblotting technique. |
Legal Information: | TWEEN is a registered trademark of Croda International PLC |
Packaging: | Foil pouches |
Reconstitution: | Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.05 M Tris buffered saline (NaCl - 0.138 M; KCl - 0.0027 M); TWEEN® 20 - 0.05%, pH 8.0, at 25 °C. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
UNSPSC | 12161700 |