Topoisomerase I human
SIGMA/T9069 - buffered aqueous glycerol solution, ≥2 units/μL
Synonym: DNA Topoisomerase I
MDL Number: MFCD03458765
Product Type: Chemical
| concentration | ≥2 units/μL |
| form | buffered aqueous glycerol solution |
| mol wt | 100 kDa |
| Quality Level | 200  |
| recombinant | expressed in baculovirus |
| storage temp. | −70°C |
| UniProt accession no. | P11387  |
| Application: | Topoisomerase I has been used in a study to assess implications for the regulation of HIV-1 replication. Topoisomerase I has also been used in a study to investigate the tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells. |
| Application: | Topoisomerase I human has been used for Topoisomerase I assay for screening inhibitors. |
| Biochem/physiol Actions: | Topoisomerase I human (TOP1) interacts with DNA by wrapping and takes a bi-lobed conformation. TOPI is essential for normal development and transcriptional regulation. Variants in the TOP1 gene is observed in patients with colorectal cancer. |
| Biochem/physiol Actions: | Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices. |
| General description: | Topoisomerase I (TOP1) is mapped to human chromosome 20q12. TOP1 is a 91 kDa type IB enzyme and consists of N-terminal highly charged domain, a core, linker domain and a C-terminal catalytic domain with Tyr-723 active site residue. |
| Physical form: | Solution containing 20 mM sodium phosphate, pH 7.4, 300 mM NaCl, 50 μg/mL BSA, 50% glycerol, and between 25-100 mM imidazole (concentration will be lot dependent). |
| Unit Definition: | One unit will relax 0.25 μg of supercoiled plasmid DNA in 30 minutes at pH 7.9 at 37 °C. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H360D |
| Precautionary statements | P201 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| Enzyme Commission (EC) Number | 5.99.1.2 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |