Anti-TRPV4 antibody produced in rabbit
SIGMA/T9075 - affinity isolated antibody, lyophilized powder
Synonym: Anti-OTRPC4; Anti-Transient Receptor Potential Channel V4
MDL Number: MFCD07370529
Product Type: Chemical
Immunohistochemistry Anti-TRPV4: Cat. No. T9075: Immunohistochemical staining of Rat dorsal root ganglion (DRG) frozen sections using Anti-TRPV4 antibody. A. TRPV4 (red) in DRG neurons. B. Staining with Mouse Anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of TRPV4 and Parvalbumin demonstrates colocalization.
Immunocytochemistry Anti-TRPV4: Cat. No. T9075: Immunocytochemical staining of paraformaldehyde-fixed and permeabilized Rat dorsal root ganglion (DRG) primary culture. A, D. Staining using Anti-TRPV4 antibody, (1:500), followed by Goat Anti-Rabbit-AlexaFluor-555 secondary antibody. B, E. Nuclear staining of cells using the cell-permeable dye Hoechst 33342. C. Merged image of panels A and B. F. Merged image of panels D and E.
Immunoblotting
Anti-TRPV4: Cat. No. T9075: Western blot analysis of ND7/23 cell line lysate.
Lanes
1. Anti-TRPV4 antibody, (1:200).
2. Anti-TRPV4 antibody, preincubated with the control peptide antigen.
Anti-TRPV4: Cat. No. T9075: Western blot analysis of ND7/23 cell line lysate.
Lanes
1. Anti-TRPV4 antibody, (1:200).
2. Anti-TRPV4 antibody, preincubated with the control peptide antigen.
Immunoblotting
Anti-TRPV4: Cat. No. T9075: Western blot analysis of Rat brain lysates.
Lanes
1. Anti-TRPV4 antibody, (1:200).
2. Anti-TRPV4 antibody, preincubated with the control peptide antigen.
Anti-TRPV4: Cat. No. T9075: Western blot analysis of Rat brain lysates.
Lanes
1. Anti-TRPV4 antibody, (1:200).
2. Anti-TRPV4 antibody, preincubated with the control peptide antigen.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | rat |
| storage temp. | −20°C |
| technique(s) | western blot: 1:200 using ND7/23 (mouse neuroblastoma X rat DRG neuron hybrid cell line) lysate |
| UniProt accession no. | Q9ERZ8  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | synthetic peptide CDGHQQGYAPKWRAEDAPL corresponding to residues 853-871 of rat TRPV4. |
| Physical form: | Lyophilized from phosphate buffered saline containing 1% BSA and 0.05% sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |