Tryptose Phosphate Broth
SIGMA/T9157 - suitable for insect cell culture
Product Type: Chemical
biological source | bovine milk |
form | solid |
Quality Level | 200 ![]() |
shipped in | ambient |
storage temp. | room temp |
technique(s) | cell culture | insect: suitable |
Application: | In addition to its use for the growth of fastidious micro-organisms, Tryptose Phospate Broth (TPB) has been studied as supplement for the preparation of media that supports vaccine production in BHK-21 cells and the growth of SF21 insect cells in high-density perfusion culture stirred-tank bioreactors. |
Application: | Tryptose Phosphate Broth has been used as a supplement: • in Eagle′s minimum essential medium alpha for growing Vero cells • for Vero-CCL81 cells in porcine epidemic diarrhea coronavirus propagation • in Dulbecco modified Eagle medium in LR7cells for mouse hepatitis virus propagation • in Glasgow minimum essential medium (GMEM) for Madin-Darby canine kidney (MDCK) cells. |
Components: | Tryptose Phosphate Broth (TPB) is composed of four components: Tryptose (20g/L); Dextrose (2g/L); NaCl (5g/L) and Disodium Phosphate (2.5g/L) typically adjusted to pH 7.3. The tryptose component is a peptone (mixture of amino acids and short peptides) derived by the mixed enzymatic hydrolysis (pancreatic enzymes) of the milk protein casein. This hydrolysate provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious mico-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity. |
General description: | Tryptose Phosphate Broth (TPB) comprises pyrimidine based components and is crucial for pyrimidine synthesis and electron transport chain in cells. In chick embryo fibroblast, it alleviates chloramphenicol inhibition. |
Packaging: | 100, 500 g in poly bottle |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | room temp |
UNSPSC | 12352200 |