Terrific Broth
SIGMA/T9179 - EZMix powder microbial growth medium
Synonym: TB media
Product Type: Chemical
A representative image of the product inner packaging
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T9179-1KG
| application(s) | agriculture food and beverages microbiology |
| description | TB; quick dissolve, dust-free formulation |
| form | powder |
| grade | Molecular Biology |
| Quality Level | 200  |
| sterility | non-sterile |
| storage temp. | room temp |
| suitability | nonselective for Escherichia coli |
| nonselective for coliforms | |
| technique(s) | microbiological culture: suitable |
| Application: | Suitable for non-selective cultivation of E. coli strains for cloning, high yield DNA plasmid production and high yield production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added. Terrific broth has been used: • as a medium for selective growth of E.coli BL21 (DE3) V2RpRARE expressing MLL SET (nuclear proto-oncogene) domain in pET28GST-LIC • to grow BL21 (DE3) cells containing plasmid expressing IL-8 (interleukin-8) • Terrific broth is also suitable for use in bacterial suspension (Bacillus subtilis strain 1085B) |
| Features and Benefits: | Terrific Broth EZMix powder provides: • Granulated, dust-free format for safer handling and faster mixing • A budget-friendly alternative to liquid • Modified formulation (without glycerol) • Easy scale-up using larger package sizes |
| General description: | Terrific Broth is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains glycerol, peptides, amino acids, water-soluble vitamins, and carbohydrates. |
| Other Notes: | *Note some of the product sizes available are given in liquid measure. This is a powder format product and those sizes reflect the final reconstituted volume. 23.6g/L Yeast Extract 11.8g/L Tryptone 9.4g/L K2HPO4 2.2g/L KH2PO4 |
| Packaging: | 1 kg in poly bottle |
| Preparation Note: | Suspend 24.1 g of powder and 4 mL of glycerol in sufficient deionized water to make 500 mL. Autoclave for 15 minutes at 121 °C to sterilize. Allow to cool before making additions, such as antibiotics (if desired). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 41106200 |