Monoclonal Anti-Tropomyosin (Sarcomeric) antibody produced in mouse
SIGMA/T9283 - clone CH1, ascites fluid
Synonym: Anti-C15orf13; Anti-CMD1Y; Anti-CMH3; Anti-HEL-S-265; Anti-HTM-alpha; Anti-LVNC9; Anti-TMSA
MDL Number: MFCD00149029
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human tongue tissue sections were stained with 1:100 Monoclonal Anti-Tropomyosin (Sarcomeric) antibody produced in Mouse, Clone: CH1(Cat. No. T9283) followed by 20 μg/mL Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat (Cat. No. B7151) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CH1, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, chicken, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using sections of human tongue |
| immunohistochemistry (frozen sections): suitable using human and animal tissue sections | |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: suitable using native preparations | |
| UniProt accession no. | P09493  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) |
| Application: | Monoclonal Anti-Tropomyosin (sarcomeric) antibody produced in mouse has been used in immunofluorescence and immunohistochemistry. |
| Biochem/physiol Actions: | Tropomyosin 1 (TPM1) is a potent tumor suppressor gene. It is expressed at low levels in various types of solid cancers including, oral squamous cell carcinoma (OSCC) and tongue squamous cell carcinoma. Mutation in the gene is associated with the development of hypertrophic cardiomyopathy. The encoded protein is involved in the regulation of actin-myosin interaction in striated muscles. Tropomyosin plays an essential role in Ca2+ -regulated contraction of cardiac muscle. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Tropomyosin 1 (TPM1) is encoded by the gene mapped to human chromosome 15q22.2. The encoded protein belongs to the TPM protein family. |
| Immunogen: | chicken muscle tropomyosin. |
| Specificity: | The antibody recognizes an epitope on skeletal and cardiac tropomyosin. The antibody shows no reaction with denatured protein by immunoblotting. No cross-reactivity has been observed with smooth muscle or non-muscle tropomyosin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |