Tris-EDTA buffer solution

SIGMA/T9285 - 100X concentrate for Northern and Southern blotting

Synonym: TE buffer solution

MDL Number: MFCD00236359
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-T9285-100ML	100 mL	$30.60 1/EA		Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

foreign activity	DNase, RNase, none detected
form	liquid
grade	for molecular biology
InChI	1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
InChI key	VLEIUWBSEKKKFX-UHFFFAOYSA-N
pH	7.8-8.2 (1 × in water)
Quality Level	200
SMILES string	NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
sterility	0.2 μm filtered
storage temp.	room temp

Application:	Suitable for storage and isolation of RNA and DNA, including purified plasmid DNA stocks. • Tris-EDTA buffer solution has been used in culture medium. • Tris-EDTA buffer solution has been used for the preparation of AmpC disks for permeabilization of Tris-EDTA to release β-lactamases from bacterial cell. • It has been used for the suspension of bacterial spores. • It has also been used as a buffer in immunohistochemistry.
Components:	TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.
General description:	TE buffer is the most commonly used buffer solution in molecular biology. "TE" is a component of Tris (a common pH buffer) and EDTA (molecule that chelate cations). TE (Tris EDTA) Buffer solubilizes DNA and RNA while protecting it from degradation. TE Buffer is used in nucleic acid isolation, which may be done prior to Northern or Southern blot hybridization.

RIDADR	NONH for all modes of transport
WGK Germany	nwg

Storage Temp.	room temp
UNSPSC	12161700

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