Thrombin human
SIGMA/T9326 - BioUltra, recombinant, expressed in HEK 293 cells, aqueous solution, ≥95% (SDS-PAGE)
Synonym: Factor IIa
MDL Number: MFCD00082072
Product Type: Chemical
| assay | ≥95% (SDS-PAGE) |
| form | aqueous solution |
| mol wt | 37.4 kDa |
| product line | BioUltra |
| Quality Level | 200 ![]() |
| recombinant | expressed in HEK 293 cells |
| shipped in | dry ice |
| specific activity | ≥2,000 units/mg protein |
| storage temp. | −70°C |
| UniProt accession no. | P00734 ![]() |
| Application: | Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess in vitro hemostatic properties of French lyophilized plasma. |
| General description: | Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. |
| Other Notes: | Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard. |
| Packaging: | 150 units in glass bottle |
| Physical form: | supplied as a solution in 20 mM MES, pH 6.0, 500 mM choline chloride |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| activity | specific activity: ≥2,000 units/mg protein |
| Storage Temp. | −70°C |
| Enzyme Commission (EC) Number | 3.4.21.5 ( BRENDA ![]() ![]() |
| UNSPSC | 12352204 |

