Thrombin human
SIGMA/T9326 - BioUltra, recombinant, expressed in HEK 293 cells, aqueous solution, ≥95% (SDS-PAGE)
Synonym: Factor IIa
MDL Number: MFCD00082072
Product Type: Chemical
The purity of Thrombin human BioUltra, recombinant (Cat. No. T9326) was assessed on SDS-PAGE stained with InstantBlue™ (Cat. No. ISB1L).
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T9326-150UN
| assay | ≥95% (SDS-PAGE) |
| form | aqueous solution |
| mol wt | 37.4 kDa |
| product line | BioUltra |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells |
| shipped in | dry ice |
| specific activity | ≥2,000 units/mg protein |
| storage temp. | −70°C |
| UniProt accession no. | P00734  |
| Application: | Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess in vitro hemostatic properties of French lyophilized plasma. |
| General description: | Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. |
| Packaging: | 150 units in glass bottle |
| Physical form: | supplied as a solution in 20 mM MES, pH 6.0, 500 mM choline chloride |
| Unit Definition: | Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| activity | specific activity: ≥2,000 units/mg protein |
| Storage Temp. | −70°C |
| Enzyme Commission (EC) Number | 3.4.21.5 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |