Trypsin inhibitor from Phaseolus limensis (lima bean)
SIGMA/T9378 - Type II-L, crude powder
Synonym: Trypsin Inhibitor from lima beans
CAS Number: 9035-81-8
EC Number: 232-906-9
MDL Number: MFCD00082102
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T9378-100MG
| biological source | Phaseolus limensis (lima bean) |
| form | crude powder |
| mol wt | 9 kDa |
| Quality Level | 200  |
| solubility | 0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL |
| storage temp. | 2-8°C |
| type | Type II-L |
| Analysis Note: | One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein. |
| Application: | Trypsin inhibitor from Phaseolus limensis (lima bean) has been used: • in the inhibition of trypsin in human plasma • in the termination of proteolytic digestion in rabbit skeletal muscles • in the inhibition of salivary glands enzyme extract from Lygus lineolaris • in the inhibition of proteolytic activity in Agave tequilana enzyme extract |
| Application: | Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes. |
| Biochem/physiol Actions: | Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains. |
| Other Notes: | View more information on Trypsin Inhibitor . |
| Packaging: | 1 g in glass bottle |
| Packaging: | 100, 500 mg in glass bottle |
| Physical properties: | Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization. |
| Preparation Note: | Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified). |
| Unit Definition: | One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 22-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |