Tris-Borate-EDTA buffer
SIGMA/T9525 - working solution
Synonym: TBE buffer
MDL Number: MFCD00236358
Product Type: Chemical
| application(s) | diagnostic assay manufacturing |
| form | working solution |
| impurities | DNase, RNase, Protease, none detected |
| InChI | 1S/C10H16N2O8.C4H11NO3.BH |
| InChI key | OSBLTNPMIGYQGY-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | OB(O)O.NC(CO)(CO)CO.OC(=O |
| sterility | 0.2 μm filtered |
| suitability | suitable for gel electrophoresis (after dilution to working concentration) |
| technique(s) | electrophoresis: suitable |
| Application: | TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution. |
| Other Notes: | 89 mM Tris borate, pH approx. 8.3, containing 2 mM EDTA. |
| Packaging: | Supplied in dispenser with a spigot. |
| Preparation Note: | Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134). |
| Preparation Note: | Solution prepared with 18 megohm water |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12161700 |