Tris Acetate-EDTA buffer
SIGMA/T9650 - 10× concentrate, BioReagent, for molecular biology, non-sterile; 0.2 μm filtered
Synonym: TAE buffer
MDL Number: MFCD00236357
Product Type: Chemical
A representative image of the product packaging
| foreign activity | Protease, none detected |
| RNAse, none detected | |
| form | solution |
| grade | for molecular biology |
| InChI | 1S/C10H16N2O8.C4H11NO3.C2 |
| InChI key | HGEVZDLYZYVYHD-UHFFFAOYSA |
| product line | BioReagent |
| Quality Level | 200  |
| SMILES string | CC(O)=O.NC(CO)(CO)CO.OC(= |
| sterility | non-sterile; 0.2 μm filtered |
| suitability | suitable for gel electrophoresis (after dilution to working concentration) |
| Application: | TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution. |
| Application: | Tris Acetate-EDTA buffer has been used for the preparation of agarose gel during DNA agarose gel electrophoresis. |
| Other Notes: | 0.4 M Tris acetate, pH approx. 8.3, containing 0.01 M EDTA. |
| Preparation Note: | Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258). |
| Preparation Note: | Solution prepared with 18 megohm water |
| Symbol |  GHS08 |
| Signal word | Warning |
| Hazard statements | H373 |
| Precautionary statements | P260 - P313 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12161700 |