Monoclonal Anti-TWEAK Receptor/Fn-14 antibody produced in mouse
SIGMA/T9700 - ~2 mg/mL, clone TR-22, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-TNF-like Weak Inducer of Apoptosis Receptor
MDL Number: MFCD07785416
Product Type: Chemical
Flow Cytometry 1×106 HT-29 cells were labeled with 2 μg/test Anti-TWEAK Receptor/Fn-14 antibody, Mouse monoclonal, Clone: TR-22 (Cat. No. T9700) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Blank histogram: negative control without first antibody; Gray histogram: antibody specific staining.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | TR-22, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~14 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | flow cytometry: suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 2-4 μg/mL using HEK-293 overexpressing TWEAKR cells lysate | |
| UniProt accession no. | Q9NP84  |
| Application: | Monoclonal Anti-TWEAK Receptor/Fn-14 antibody produced in mouse is suitable for: • enzyme-linked immunosorbent assay (ELISA) • immunoblotting • flow cytometry |
| Biochem/physiol Actions: | Fn14 induces growth effects in neurons independent of the ligand, TWEAK. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-TWEAK Receptor (mouse IgG1 isotype) is derived from the TR-22 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with human TWEAK receptor. Tumor necrosis factor receptor superfamily member 12A or TNF-like weak inducer of apoptosis Receptor (TWEAKR) or fibroblast growth factor-inducible immediate-early response protein 14 (fn14) is a type I transmembrane receptor comprising transmembrane domain, amino-terminal signal peptide, and carboxy-terminal cytoplasmic domain. The TWEAKR gene is mapped to human chromosome 16p13.3. TWEAKR is expressed in the placenta, lungs, kidney, and heart. |
| Immunogen: | synthetic peptide corresponding to amino acids 39-52 in the N-terminus of human TWEAK receptor. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Monoclonal Anti-TWEAK receptor recognizes human TWEAK receptor |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recom-mended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |