TRAIL human
SIGMA/T9701 - recombinant, expressed in E. coli, ≥98% (SDS-PAGE and HPLC), lyophilized powder
Synonym: Apo2 Ligand; Apo2L; TL2; TNF-related apoptosis-inducing ligand
CAS Number: 2244730-93-4
MDL Number: MFCD01867158
Product Type: Chemical
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| assay | ≥98% (SDS-PAGE and HPLC) |
| biological source | human |
| description | extracellular domain |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | 19.6 kDa |
| predicted mol wt ~21 kDa | |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: suitable |
| UniProt accession no. | P50591  |
| Analysis Note: | The biological activity is determined by its ability to induce apoptotic cell death in TRAIL-sensitive LN-18 cells. |
| Application: | Human TRAIL can be used as a selection agent for treating human cells for phenotypic enrichments. The product can also be used in chemical transfection for identifying Small RNA Modulators of TRAIL-Induced Apoptosis Pathway by utilizing libraries of synthetic small interfering RNA (siRNA) and microRNAs (miRNA). |
| Application: | TRAIL human has been used in immunofluorescence analyses, to study the delocalization of melanoma antigen-D2 in the nucleoplasm after a cellular stress. |
| Biochem/physiol Actions: | TNF-related apoptosis-inducing ligand (TRAIL) is a type II transmembrane protein that belongs to TNF family. The product present in both soluble as well as membrane-bound form, can induce rapid apoptosis of various transformed cell lines but not the normal cells. Additionally, the product can also induce apoptotic cell death in TRAIL-sensitive U343MG cells. |
| Biochem/physiol Actions: | TRAIL is a cytokine with anti-inflammatory, and antitumor activity. It is expressed in various cells including immune cells, vascular cells and adipocytes. TRAIL is associated with vascular endothelial cell protection. |
| Biochem/physiol Actions: | Type II transmembrane protein of the TNF superfamily. Ligand for apoptosis-inducing death receptors DR4 and DR5, decoy receptors DcR1 and DcR2, and osteoprotegerin. Approximately 28% amino acid sequence identity to FAS ligand. Human and mouse TRAIL share 65% sequence identity. |
| Physical form: | Lyophilized from phosphate buffered saline |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE and HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |