Tricine
SIGMA/T9784 - BioXtra, pH 4.0-6.0 (1 M in H2O), ≥99% (titration)
Synonym: N-
CAS Number: 5704-04-1
Empirical Formula (Hill Notation): C6H13NO5
Molecular Weight: 179.17
EC Number: 227-193-6
MDL Number: MFCD00004277
Linear Formula: (HOCH2)3CNHCH2CO2H
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T9784-100G
This image depicts SKU: T9784-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T9784-250G
This image depicts SKU: T9784-250G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T9784-1KG
This image depicts SKU: T9784-1KG
| absorption | ≤0.05 at 280 in H2O at 1 M |
| ≤0.1 at 260 in H2O at 1 M | |
| anion traces | chloride (Cl-): ≤0.1% |
| sulfate (SO42-): ≤0.05% | |
| application(s) | diagnostic assay manufacturing |
| assay | ≥99% (titration) |
| cation traces | Al: ≤0.0005% |
| Ca: ≤0.0005% | |
| Cu: ≤0.0005% | |
| Fe: ≤0.0005% | |
| K: ≤0.005% | |
| Mg: ≤0.0005% | |
| Na: ≤0.005% | |
| NH4+: ≤0.05% | |
| Pb: ≤0.001% | |
| Zn: ≤0.0005% | |
| form | powder |
| ign. residue | ≤0.1% |
| impurities | ≤0.005% Phosphorus (P) |
| ≤0.1% Insoluble matter | |
| InChI | 1S/C6H13NO5/c8-2-6(3-9,4- |
| InChI key | SEQKRHFRPICQDD-UHFFFAOYSA |
| pH | 4.0-6.0 (1 M in H2O) |
| pKa (25 °C) | 8.1 |
| product line | BioXtra |
| Quality Level | 400  |
| SMILES string | OCC(CO)(CO)NCC(O)=O |
| solubility | H2O: 1 M, clear, colorless |
| useful pH range | 7.4-8.8 |
| Application: | Buffer component for separation of low molecular weight peptides. |
| Application: | Tricine has been used: • in a polymerase chain reaction (PCR) buffer used in the single-cell collection • as a component of lactotroph feeding medium for culturing lactotrophs • to prepare luciferase assay reagent for luciferase activity assay in firefly Photonis pyralis and as a component in Triton/glycylglycine lysis buffer |
| General description: | The name Tricine is derived from "tris" and "glycine". Tricine and other good buffers serve as efficient scavengers of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is widely used as an electrophoresis buffer and for the resuspension of cell pellets. Due to its lower negative charge compared to glycine, it migrates faster. It has a high ionic strength which leads to more ion movement and less protein movement. This aids the separation of low molecular weight proteins in lower percent acrylamide gels. Tricine is also used as a trailing ion that permits the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems. |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 100, 250 g in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (titration) |
| UNSPSC | 12161700 |