Trizma® Pre-set crystals
SIGMA/T9818 - BioPerformance Certified, pH 9.1, average Mw 123.0
Synonym: Tris crystals
Product Type: Chemical
| application(s) | diagnostic assay manufacturing |
| form | crystalline powder |
| grade | BioPerformance Certified |
| impurities | DNase, RNase, Protease, NICKase, none detected |
| endotoxin, tested | |
| total aerobic microbial count, tested | |
| ≤0.05 A290 | |
| ≤0.5% water (Karl Fischer) | |
| ≤5 ppm Heavy metals (as Pb) | |
| mol wt | average Mw 123.0 |
| pH | 9.1 |
| 9.10 (25 °C, 0.1 M)(lit.) | |
| Quality Level | 200  |
| solubility | water: 0.667 g/mL, clear, colorless |
| technique(s) | cell culture | mammalian: suitable |
| useful pH range | 7.0-9.0 |
| Application: | Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units. Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature. From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C. From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C. If a different concentration is desired, note that there is a concentration effect on the pH of the solution. |
| Application: | The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases. |
| Legal Information: | Trizma is a registered trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Certain pH electrodes do not give accurate results with Tris buffers. |
| Other Notes: | This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer. |
| Packaging: | 100, 250 g in poly bottle |
| Preparation Note: | Trizma solutions can be autoclaved. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315-H319-H335 |
| Precautionary statements | P261-P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 2 |
| UNSPSC | 12161700 |