Anti-Tubulin, Polyglutamylated antibody, Mouse monoclonal
SIGMA/T9822 - clone B3, purified from hybridoma cell culture
Synonym: Anti-K-ALPHA-1
MDL Number: MFCD03792435
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-Tubulin, Polyglutamylated Clone: B3 (Cat. No. T9822). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. SH-SY5Y 2. Rat Brain 3. Mouse Brain
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | B3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgM |
| mol wt | antigen ~50 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, sea urchin (Lytechinus pictus and three others.), bovine, monkey, rabbit, human, rat, Oxyrrhis marina (dinoflagellate), chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.5-1 μg/mL using cytosolic fraction of rat brain extract | |
| UniProt accession no. | P68363  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunocytochemistry (1 paper) Western Blotting (1 paper) |
| Application: | Monoclonal anti-tubulin, polyglutamylated antibody can be used in immunoblotting, immunocytochemistry and ELISA. It can also be used as primary antibodies (diluted 1:1000) in western blotting. |
| Application: | Monoclonal Anti-Tubulin, Polyglutamylated antibody produced in mouse has been used in: • immunoprecipitation • immunohistochemistry • immunofluorescence |
| Biochem/physiol Actions: | Microtubule plays an important role in DNA segregation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Tubulin is a 100kD protein that is present in all cells as a heterodimer of two similar polypeptides α and β which assemble to form microtubules. Proper organization of microtubules is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-tubulin, polyglutamylated antibody can be used as primary antibody in colocalization experiments. This antibody can also be used for studies involving the inhibition of flagellar motility in permeabilized sperm models. Mouse anti-tubulin, polyglutamylated antibody reacts specifically with the epitope present in the C-terminal region of α and β tubulins (glutamylated motif at amino acids 445-457 of α-tubulin). |
| General description: | Tubulin is a cellular colchicine-binding protein. |
| Immunogen: | purified sea urchin (Lytechinus pictus) sperm axonemal proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |