ThermaGo™
SIGMA/TGO - increases PCR specificity, inhibits non-specific polymerase activity, prevents mis-priming during PCR
Synonym: Multiplex PCR; PCR Additive; hot start DNA polymerase; hot start PCR; hotstart
Product Type: Chemical
ThermaStop™ is a modified nucleic acid that acts as a PCR additive that increases PCR specificity, inhibits non-specific polymerase activity, prevents mis-priming before PCR.
| color | opaque |
| form | lyophilized powder |
| packaging | pkg of One unit of ThermaGo™ is defined as the amount required for maximum improvements in amplification reactions containing 1 unit of Taq DNA polymerase in a volume of 25 μl. |
| units of 2500 units | |
| units of 500 units | |
| Quality Level | 100  |
| shipped in | ambient |
| storage temp. | 2-8°C |
| technique(s) | PCR: suitable |
| Application: | ThermaGo™ is suitable for: • monoplex and multiplexed end-point single nucleotide polymorphism (SNP) genotyping • detection of low-copy target numbers during single-cell PCR and digital PCR • detection of low-copy target numbers in liquid biopsies/tumor samples • enhancing the accuracy of multiplex PCR for next-generation sequencing and other post PCR applications |
| Features and Benefits: | • Acts at higher temperatures during annealing and elongation steps • Improves the reproducibility • Detects low-copy number targets by suppressing non-specific targets • Minimizes signal scatter in digital & standard PCR • Minimizes mispriming and chimera production before next-generation sequencing (NSG) • Enhances end-point genotyping |
| General description: | ThermaGo™ is a double-stranded, modified nucleic acid that terminates the mispriming by interacting with DNA polymerase during the annealing and extension steps of PCR. It improves the specificity of Taq DNA polymerase. ThermaStop™ and ThermaGo act together during PCR amplification at different temperatures to increase specificity and prevent errors. |
| Legal Information: | ThermaGo is a trademark of ThermaGenix Inc. |
| Legal Information: | ThermaStop is a trademark of ThermaGenix Inc. |
| Reconstitution: | Dissolve in Tris pH 8.3 buffer |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |