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In Vitro Toxicology Assay Kit, MTT based

SIGMA/TOX1

Synonym: mitochondrial activity assay

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-TOX1-1KT 1 kit
 $536.00
1/EA		 Add To Favorites
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λmax 570 nm
application(s) cell analysis
detection
detection method colorimetric
packaging pkg of 1 kit
Quality Level 200 
storage condition dry at room temperature
storage temp. 2-8°C
usage  kit sufficient for 1,000 tests
Application: In Vitro Toxicology Assay Kit, MTT based has been used to perform an (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) MTT assay to measure the cytotoxicity of gold nanoparticles (AuNP). It has also been used to examine cell viability in SH-SY5Y cultures in response to 6-hydroxydopamine (6-OHDA) treatment.
Biochem/physiol Actions: Conversion of MTT to a water-insoluble colored formazan derivative which is then solubilized in acidic isopropanol.
General description: This kit is designed for determining cell number/ cell viability spectrophotometrically as a function of mitochondrial activity in living cells. The MTT method is simple, accurate and yields reproducible results. The key component is (3- [4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) or MTT. Solutions of MTT, dissolved in medium or balanced salt solutions without phenol red, are yellowish in color. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, yielding purple formazan crystals which are insoluble in aqueous solutions. Absorbance of converted dye is measured at a wavelength of 570nm.
Storage Temp. 2-8°C
UNSPSC 12352207

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