In Vitro Toxicology Assay Kit, Neutral Red based
SIGMA/TOX4
Synonym: NR uptake assay; Neutral red assay
Product Type: Chemical
λmax | 540 nm |
application(s) | cell analysis detection |
detection method | colorimetric |
form | liquid |
packaging | pkg of 1 kit |
Quality Level | 200 ![]() |
storage condition | dry at room temperature |
storage temp. | 2-8°C |
usage | kit sufficient for 1,000 tests |
Application: | In Vitro Toxicology Assay Kit, Neutral Red based has been used: • to measure the lysosomal enzyme activity in HB8902 cells • to study the cell viability of human alveolar epithelial cells • to study the cell viability in fibroblasts |
Biochem/physiol Actions: | Neutral red (NR) is a red dye that accumulates in the lysosome by actively transferring across the cell membranes. The dye is extracted and the uptake is quantitated by spectroscopy. The NR uptake assay is the most commonly used cytotoxicity test. It is widely applicable in environmental and biomedical studies to estimate the number of viable cells in culture. The NR dye in vitro tissue culture studies is used in assessing viral cytopathogenicity and detection of toxic compounds. |
General description: | For spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 540 nm. |
Symbol | ![]() ![]() ![]() |
Signal word | Danger |
Hazard statements | H226 - H317 - H319 - H350 |
Precautionary statements | P202 - P210 - P280 - P303 + P361 + P353 - P305 + P351 + P338 - P308 + P313 |
Hazard Codes | Xi |
Risk Statements | 10-43 |
Safety Statements | 36/37 |
RIDADR | UN 3316 9 |
Flash Point(F) | 73.4 °F |
Flash Point(C) | 23 °C |
Storage Temp. | 2-8°C |
UNSPSC | 12352200 |