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In Vitro Toxicology Assay Kit, Neutral Red based

SIGMA/TOX4

Synonym: NR uptake assay; Neutral red assay

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-TOX4-1KT 1 kit
$359.00
1/EA
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λmax 540 nm
application(s) cell analysis
detection
detection method colorimetric
form liquid
packaging pkg of 1 kit
Quality Level 200 
storage condition dry at room temperature
storage temp. 2-8°C
usage  kit sufficient for 1,000 tests
Application: In Vitro Toxicology Assay Kit, Neutral Red based has been used:
• to measure the lysosomal enzyme activity in HB8902 cells
• to study the cell viability of human alveolar epithelial cells
• to study the cell viability in fibroblasts

Biochem/physiol Actions: Neutral red (NR) is a red dye that accumulates in the lysosome by actively transferring across the cell membranes. The dye is extracted and the uptake is quantitated by spectroscopy. The NR uptake assay is the most commonly used cytotoxicity test. It is widely applicable in environmental and biomedical studies to estimate the number of viable cells in culture. The NR dye in vitro tissue culture studies is used in assessing viral cytopathogenicity and detection of toxic compounds.
General description: For spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 540 nm.
Storage Temp. 2-8°C
UNSPSC 12352200

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