In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based
SIGMA/TOX7
Synonym: LDH cytotoxicity assay
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| λmax | 490 nm |
| application(s) | cell analysis detection |
| detection method | colorimetric |
| form | liquid |
| packaging | pkg of 1 kit |
| Quality Level | 200  |
| storage condition | dry at room temperature |
| storage temp. | −20°C |
| technique(s) | protein quantification: suitable |
| usage | 1 kit sufficient for 500 tests |
| Application: | In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based has been used: • to detect lactate dehydrogenase release in human umbilical vein endothelial cells (HUVECs) and human cardiac myocytes (HCMs) for lactate dehydrogenase (LDH) assay • to determine the activity of lactate dehydrogenase leakage of MDA-MB-231 cells • to examine the activity of LDH in RAW 264.7 cells , in LDH cytotoxicity assay in human cell lines • for spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 490 nm. |
| Biochem/physiol Actions: | The lactate dehydrogenase assay is a means of measuring either the number of cells via total cytoplasmic lactate dehydrogenase (LDH) or membrane integrity as a function of the amount of cytoplasmic LDH released into the medium. This method is simple, accurate and yields reproducible results. The assay is based on the reduction of NAD by LDH. The resulting reduced NAD (NADH) is utilized in the stoichiometric conversion of a tetrazolium dye to a soluble, colored formazan derivative. . |
| General description: | Tox7 is a member of Sigma’s CytoToxicity Kit series. It is based on the measurement of lactate dehydrogenase (LDH) release from cells as a measure of cell membrane damage and cytotoxicity. This technique has been utilized as an alternative to 51Cr release for cell mediated cytotoxicity assays, as well as conventional cytotoxicity resulting from interaction of a test material with the cell. |
| Hazard statements | H412 |
| Precautionary statements | P273 - P501 |
| Hazard Codes | Xi |
| Risk Statements | 36-52/53 |
| Safety Statements | 26-61 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | −20°C |
| UNSPSC | 12352207 |