FLAG® HA Tandem Affinity Purification Kit
SIGMA/TP0010
Synonym: Anti-ddddk; Anti-dykddddk
Product Type: Chemical
| Quality Level | 200 ![]() |
| storage temp. | 2-8°C |
| Application: | The FLAG® HA Tandem Affinity Purification Kit is designed for the isolation of high purity FLAG-HA dual-tagged fusion proteins from complex matrix, such as cell lysates and tissue homogenates. The kit is particularly suitable for isolation of protein complexes using TAP (Tandem Affinity Purification) technology. Sutitable for mass spectrometry analysis and western blotting. Learn more product details in our FLAG® application portal. |
| General description: | TAP technology is a recent development in protein purification. This technology incorporates tandem-linked affinity tags into genes of interest so that high purity fusion proteins can be isolated through two consecutive affinity purification steps. The technology is particularly useful for the isolation and identification of protein complexes by adding a tandem affinity tag to a known "bait" protein to pull down endogenous proteins that interact with the targeted protein of interest. |
| Legal Information: | FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Symbol | ![]() GHS07,GHS09 |
| Signal word | Warning |
| Hazard statements | H319 - H411 |
| Precautionary statements | P264 - P273 - P280 - P305 + P351 + P338 - P337 + P313 - P391 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |
| Components | EZview™ Red ANTI-FLAG® M2 Affinity Gel, clone M2; Monoclonal Anti-HA−Agarose antibody produced in mouse, clone HA-7, purified immunoglobulin, PBS suspension; RIPA Buffer; 3X FLAG® Peptide, lyophilized powder; Urea, 8 M (after reconstitution with 16 mL high purity water) |



