SygRNA™ Cas9 Synthetic tracrRNA
SIGMA/TRACRRNA05N - SygRNA™ SpCas9 tracrRNA
Synonym: RNA scaffold
Product Type: Chemical
SygRNA™ synthetic Francisella novicida tracrRNA complexed with SygRNA™ synthetic Francisella novicida crRNA. For use with MISSION® FnCas9 proteins in cell-free applications.
| packaging | pkg of 5 nmol × Tube (HPLC) |
| shipped in | ambient |
| storage temp. | −20°C |
| Application: | Functional Genomics/Transgenic Applications • Creation of gene knockouts • Creation of knock-in animals or cell lines with promoters, fusion tags or reporters integrated into endogenous genes |
| Features and Benefits: | HPLC-purified SygRNA™ Cas9 tracrRNA oligo is optimized for use with a target-specific cRNA and a source of SpCas9 protein for genome editing applications. |
| General description: | The SygRNA™ synthetic RNA system recruits SpCas9 nuclease to specific genomic targets. The tracrRNA (trans-activating crRNA) binds to a crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides Cas9 protein to crRNA-specified sites in the genome. SygRNA™ synthetic tracrRNA and crRNAs are optimized for use with Cas9 expressing of cell lines, Cas9 protein, or Cas9 mRNA and compatible with a variety of delivery methods such as microinjection, lipofection, and electroporation. |
| Legal Information: | CRISPR Use License Agreement  |
| Legal Information: | SygRNA is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | The SygRNA™ Cas9 tracrRNA oligo is used in conjunction with a target-specific crRNA and a source of Cas9 protein such as TGEN-CP-50UG, CAS9P, or CAS9MRNA to form active RNP complexes. Select predesigned unique crRNAs against human, mouse and rat genes at CRISPR search . Learn more about SygRNA™ tracrRNA Need custom tracrRNA ? |
| Physical form: | Dry |
| Preparation Note: | Resuspend SygRNA™ Cas9 tracrRNA oligo in a RNAase-free Tris-containing buffer with a pH 8.0 or less. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106609 |