TrueGel3D Hydrogel Kits
SIGMA/TRUE2 - FAST-DEXTRAN, allow cell recovery, PEG non cell-degradable crosslinker
Product Type: Chemical
Cyst formation of MDCK cells in TrueGel hydrogel
| shipped in | dry ice |
| storage temp. | −70°C |
| Application: | TRUE2-1KT is used to grow cells that don′t attach to hydrogel for survival but rather use their secreted extracellular matrix. It is used to immobilize cells or specimens for imaging and for bioprinting. |
| Disclaimer: | Avoid prolong exposure of PEG non cell-degradable crosslinker to air. |
| Features and Benefits: | • Fast gelling (takes few seconds to minutes after addition of crosslinker) • Allow cell recovery and post-culture analyses • Flexibility in modulating gel stiffness • Cells do not attach to hydrogel (if not customized with TrueGel3D RGD peptide) • Does not allow cell migration |
| General description: | The gel is formed by stable thioether bonds between maleimide groups of dextran (FAST-DEXTRAN) and thiol groups on PEG non cell-degradable crosslinker. Maleimide-functionalized dextran is specifically used to decrease the time for gel formation. The dextran polymer does not have cell attachment sites, but it can be customized to conjugate RGD peptide using TrueGel3D RGD (TRUERGD-1UM) prior to the crosslinking step. TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies. |
| Other Notes: | TRUE2-1KT contains lyophilized FAST-DEXTRAN (polymer) and PEG non cell-degradable crosslinker with reconstitution buffer, TrueGel3D thioglycerol, and water. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 |
| Precautionary statements | P234 - P390 |
| RIDADR | UN 1789 8 / PGIII |
| Storage Temp. | −70°C |
| UNSPSC | 12352207 |