TrueGel3D Hydrogel Kits
SIGMA/TRUE6 - SLO-DEXTRAN, allow cell recovery, PEG non cell-degradable crosslinker
Synonym: 3D Hydrogel Kit; TrueGel3D Kit
Product Type: Chemical
Cyst formation of MDCK cells in TrueGel hydrogel
| shipped in | dry ice |
| storage temp. | −70°C |
| Application: | TRUE6-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic natural tissue stiffness and to investigate the effect of mechanical stimulus on cell physiology and behavior. It is also used to culture non-proliferating cells and proliferating spheroids that do not attach to hydrogel for survival, but rather use their own extracellular matrix. |
| Disclaimer: | Avoid prolong exposure of PEG non cell-degradable crosslinker to air. |
| Features and Benefits: | • Slow gelling (takes 8 minutes to 25 minutes, depends on gel stiffness) • Flexibility in modulating gel stiffness • Does not allow cell migration • Allow cell recovery and post-culture analyses • Cell inert hydrogel (if not customized with RGD peptide) |
| General description: | The gel is formed by stable thioether bonds between thiol groups on PEG non cell-degradable crosslinker and thio-reactive groups on SLO-DEXTRAN. The polymer, SLO-DEXTRAN is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-DEXTRAN. SLO-DEXTRAN does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step. TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies. |
| Other Notes: | TRUE6-1KT contains SLO-DEXTRAN solution in phosphate buffer and lyophilized PEG non cell-degradable crosslinker with reconstitution buffer and water. |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 - H315 - H319 |
| Precautionary statements | P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −70°C |
| UNSPSC | 12352207 |