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TrueGel3D Polymer

SIGMA/TRUEDEXF - Modified for fast gelling (FAST-DEXTRAN),allow cell recovery

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-TRUEDEXF-170UL 170 µL
 $235.00
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Cyst formation of MDCK cells in TrueGel hydrogel

 

shipped in dry ice
storage temp. −70°C
Application: FAST-DEXTRAN polymer is used with crosslinkers (CD/PEG crosslinkers) to establish stable hydrogels
Disclaimer: Avoid frequent freeze-thaw cycles and store FAST-DEXTRAN in small portions
Features and Benefits: • Contains dextrose polymer functionalized with maleimide groups for quick gelation
• Degradable polymer
General description: Dextrose polymers are functionalized with thiol-reactive maleimide groups, which react rapidly with thiol groups of crosslinkers to form hydrogels in few seconds. The thiol-reactive maleimide groups are also compatible to conjugate polymers with bioactive factors. The thioglycerol is used to maintain hydrogel strength and also as a control to compare cellular behavior in peptide-modified versus unmodified hydrogels. The buffers (pH7.2 and pH 5.5) are used to control pH and osmotic conditions to optimize time for gelation.

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.
Other Notes: TRUEDEXF contains FAST-DEXTRAN lyophilized with reconstitution buffer, TrueGel3D thioglycerol and water
Storage Temp. −70°C
UNSPSC 12352207

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