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TrueGel3D Polymer

SIGMA/TRUEDEXS - Modified for slow gelling (SLO-DEXTRAN), allow cell recovery

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-TRUEDEXS-170UL 170 µL
 $235.00
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Cyst formation of MDCK cells in TrueGel hydrogel

 

shipped in dry ice
storage temp. −70°C
technique(s) cell attachment: suitable
Application: SLO-DEXTRAN polymer is used with crosslinkers (CD/PEG crosslinkers) to establish stable hydrogels
Disclaimer: Avoid frequent freeze-thaw cycles and store SLO-DEXTRAN in small portions
Features and Benefits: • Contains dextrose polymer functionalized with thiol reactive groups for slow gelation
• Degradable polymer
General description: Dextrose polymers are functionalized with thiol-reactive groups, which react at a slower rate with thiol groups of crosslinkers (CD/PEG crosslinkers) to form stable hydrogels. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of homogenous gel formation. The thioglycerol is used to maintain hydrogel strength and also as a control to compare cellular behavior in peptide-modified versus unmodified hydrogels.

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.
Other Notes: TRUEDEXS contains SLO-DEXTRAN solution in phosphate buffer, TrueGel3D thioglycerol and water
Storage Temp. −70°C
UNSPSC 12352207

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