Synonym: 5Z,8Z,11Z,14Z-Eicosatetraenoic acid 2-oxo-2H-1-benzopyran-7-yl ester; 7-Hydroxycoumarinyl Arachidonate
CAS Number: 161180-11-6
Empirical Formula (Hill Notation): C29H36O4
Molecular Weight: 448.59
EC Number: 200-578-6
MDL Number: MFCD00797670
Linear Formula: C29H36O4
Product Type: Chemical
assay |
≥98% (HPLC) |
biological source |
synthetic (organic) |
form |
ethanol solution |
InChI |
1S/C29H36O4/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-28(30)32-26-22-20-25-21-23-29(31)33-27(25)24-26/h6-7,9-10,12-13,15-16,20-24H,2-5,8,11,14,17-19H2,1H3/b7-6-,10-9-,13-12-,16-15- |
InChI key |
SFTGFGOBCQCZDY-DOFZRALJSA-N |
Quality Level |
200 ![External link](https://krackeler.com/images/sigma/ext-link.gif) |
shipped in |
wet ice |
SMILES string |
CCCCCC=C/CC=C/CC=C/CC=C/CCCC(=O)Oc1ccc2C=CC(=O)Oc2c1 |
solubility |
aqueous buffer: slightly soluble |
|
DMF: soluble 50 mg/mL |
|
DMSO: soluble 25 mg/mL |
storage temp. |
−20°C |
technique(s) |
immunofluorescence: suitable |
Application: |
Umbelliferyl Arachidonate also known as 7-Hydroxycoumarinyl Arachidonate was used as a fluorogenic substrate for developing a fluorescence-based assay to evaluate the inhibitors against human recombinant monoacylglycerol lipase. |
Biochem/physiol Actions: |
Monoacylglycerol lipase (MAGL) protein facilitates the hydrolysis of Umbelliferyl Arachidonate and generates arachidonic acid and the highly fluorescent 7-hydroxyl coumarin (7-HC). Hence, it acts as a marker in a fluorescence-based assay. |
Preparation Note: |
Umbelliferyl Arachidonate can be dissolved in DMSO at 25 mg/ml and in DMF at 50 mg/ml. The product is sparingly soluble in aqueous buffers. |
Symbol |
![GHS02 GHS02](https://krackeler.com/images/sigma/GHS02.gif) GHS02,GHS07 |
Signal word |
Danger |
Hazard statements |
H225 - H319 |
Precautionary statements |
P210 - P305 + P351 + P338 |
Hazard Codes |
F |
Risk Statements |
11 |
Safety Statements |
7-16 |
RIDADR |
UN1170 - class 3 - PG 2 - Ethanol, solution |
WGK Germany |
WGK 2 |
Flash Point(F) |
57.2 °F - closed cup |
Flash Point(C) |
14 °C - closed cup |
Purity |
≥98% (HPLC) |
Storage Temp. |
−20°C |
UNSPSC |
41106300 |