Urea
SIGMA/U0631 - BioXtra, pH 7.5-9.5 (20 °C, 5 M in H2O)
Synonym: Carbamide; Carbonyldiamide
CAS Number: 57-13-6
Empirical Formula (Hill Notation): CH4N2O
Molecular Weight: 60.06
EC Number: 200-315-5
MDL Number: MFCD00008022
Linear Formula: NH2CONH2
Product Type: Chemical
absorption | ≤0.06 at 260 nm at 5 M |
≤0.06 at 280 nm at 5 M | |
anion traces | chloride (Cl-): ≤0.05% |
sulfate (SO42-): ≤0.05% | |
application(s) | diagnostic assay manufacturing |
assay | 98.5-101.5% |
cation traces | Al: ≤0.0005% |
Ca: ≤0.001% | |
Cu: ≤0.0005% | |
Fe: ≤0.0005% | |
K: ≤0.005% | |
Mg: ≤0.0005% | |
Na: ≤0.005% | |
NH4+: ≤0.05% | |
Pb: ≤0.001% | |
Zn: ≤0.0005% | |
density | 1.335 g/mL at 25 °C (lit.) |
form | powder, crystals or pellets |
ign. residue | ≤0.1% |
impurities | ≤0.005% Phosphorus (P) |
≤0.1% Insoluble matter | |
InChI | 1S/CH4N2O/c2-1(3)4/h(H4,2 |
InChI key | XSQUKJJJFZCRTK-UHFFFAOYSA |
mp | 132-135 °C (lit.) |
pH | 7.5-9.5 (20 °C, 5 M in H2O) |
product line | BioXtra |
Quality Level | 200 ![]() |
SMILES string | NC(N)=O |
solubility | H2O: 5 M at 20 °C, clear, colorless |
Application: | Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. |
Packaging: | 1 kg in poly bottle |
Packaging: | 5 kg in poly drum |
Packaging: | 500 g in poly bottle |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | 98.5-101.5% |
mp | 132-135 °C (lit.) |
Density | 1.335 g/mL at 25 °C (lit.) |
UNSPSC | 12352200 |