Urea
SIGMA/U0631 - BioXtra, pH 7.5-9.5 (20 °C, 5 M in H2O)
Synonym: Carbamide; Carbonyldiamide
CAS Number: 57-13-6
Empirical Formula (Hill Notation): CH4N2O
Molecular Weight: 60.06
EC Number: 200-315-5
MDL Number: MFCD00008022
Linear Formula: NH2CONH2
Product Type: Chemical
| absorption | ≤0.06 at 260 nm at 5 M |
| ≤0.06 at 280 nm at 5 M | |
| anion traces | chloride (Cl-): ≤0.05% |
| sulfate (SO42-): ≤0.05% | |
| application(s) | diagnostic assay manufacturing |
| assay | 98.5-101.5% |
| cation traces | Al: ≤0.0005% |
| Ca: ≤0.001% | |
| Cu: ≤0.0005% | |
| Fe: ≤0.0005% | |
| K: ≤0.005% | |
| Mg: ≤0.0005% | |
| Na: ≤0.005% | |
| NH4+: ≤0.05% | |
| Pb: ≤0.001% | |
| Zn: ≤0.0005% | |
| density | 1.335 g/mL at 25 °C (lit.) |
| form | powder, crystals or pellets |
| ign. residue | ≤0.1% |
| impurities | ≤0.005% Phosphorus (P) |
| ≤0.1% Insoluble matter | |
| InChI | 1S/CH4N2O/c2-1(3)4/h(H4,2 |
| InChI key | XSQUKJJJFZCRTK-UHFFFAOYSA |
| mp | 132-135 °C (lit.) |
| pH | 7.5-9.5 (20 °C, 5 M in H2O) |
| product line | BioXtra |
| Quality Level | 200 ![]() |
| SMILES string | NC(N)=O |
| solubility | H2O: 5 M at 20 °C, clear, colorless |
| Application: | Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 5 kg in poly drum |
| Packaging: | 500 g in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 98.5-101.5% |
| mp | 132-135 °C (lit.) |
| Density | 1.335 g/mL at 25 °C (lit.) |
| UNSPSC | 12352111 |

